Expression of inflammation-associated genes in circulating leukocytes and activity of indoleamine-2,3-dioxygenase in dairy cattle with acute puerperal metritis and bacteremia

被引：7

作者：

Credille, Brent C. ^{[1
]}

Woolums, Amelia R. ^{[2
]}

Overton, Michael W. ^{[1
]}

Hurley, David J. ^{[1
]}

Giguere, Steeve ^{[2
]}

机构：

[1] Univ Georgia, Coll Vet Med, Dept Populat Hlth, Athens, GA 30602 USA

[2] Univ Georgia, Coll Vet Med, Dept Large Anim Med & Surg, Athens, GA 30602 USA

来源：

RESEARCH IN VETERINARY SCIENCE | 2015年 / 101卷

关键词：

Uterine; Blood culture; Cytokine; Metritis; Dairy; Polymerase chain reaction; COMPENSATORY ANTIINFLAMMATORY RESPONSE; POSTPARTUM UTERINE DISEASE; BLOOD MONONUCLEAR-CELLS; INDOLEAMINE 2,3-DIOXYGENASE; SEVERE SEPSIS; T-CELLS; COWS; DIFFERENTIATION; NEUTROPHILS; TRYPTOPHAN;

D O I：

10.1016/j.rvsc.2015.05.013

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Objective: To investigate whether expression of genes associated with inflammation and activity of indoleamine-2,3-dioxygenase (IDO) correlated with disease status and prevalence of bacteremia in post-partum dairy cattle with and without acute puerperal metritis (APM). Procedures: Blood was collected from cattle with APM and control cattle matched by parity and days in milk. Leukocytes were isolated and expression of 6 genes was quantified. Activity of IDO was measured in serum with higher performance liquid chromatography (HPLC). Results: The relative expression of IL-1 beta in cattle with APM was significantly lower than that in controls. IDO activity was not significantly different between bacteremic and non-bacteremic cattle Conclusions and clinical relevance: The expression of IL-1 beta was lower in cattle with APM. The lower levels of IL-1 beta expression in PBMCs of cattle with APM suggest impaired inflammatory responses and may contribute to the development of the disease in this population of animals. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：6 / 10

页数：5

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