Characterization of an antibody-drug conjugate by hydrophilic interaction chromatography coupled to mass spectrometry

被引：40
作者：
D'Atri, Valentina [1 ]
Fekete, Szabolcs [1 ]
Stoll, Dwight [2 ]
Lauber, Matthew [3 ]
Beck, Alain [4 ]
Guillarme, Davy [1 ]
机构：
[1] Univ Geneva, Univ Lausanne, CMU, Sch Pharmaceut Sci, Rue Michel Servet 1, CH-1211 Geneva 4, Switzerland
[2] Gustavus Adolphus Coll, Dept Chem, St Peter, MN 56082 USA
[3] Waters Corp, 34 Maple St, Milford, MA 01757 USA
[4] Ctr Immunol Pierre Fabre, 5 Ave Napoleon III,BP 60497, St Julien En Genevois, France
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2018年 / 1080卷
基金：
瑞士国家科学基金会;
关键词：
Antibody-drug conjugate; Hydrophilic interaction chromatography; High-resolution mass spectrometry; Post-translational modifications; Glycans profiling; STRUCTURAL-CHARACTERIZATION; POSITIONAL ISOMERS; SERUM CLEARANCE; MIDDLE-UP; N-GLYCANS; IGG1; INTACT;
D O I：
10.1016/j.jchromb.2018.02.026
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Brentuximab vedotin (Adcetris) is a cysteine-linked antibody-drug conjugate (ADC) used in the treatment of Hodgkin lymphoma (HL) and systemic anaplastic large cell lymphoma (ALCL). In this study, the drug payload and glycan modifications of this ADC were simultaneously characterized using a unique LC-MS middle-up analysis, involving hydrophilic interaction chromatography (HILIC). This work demonstrates that HILIC is an effective and complementary analytical technique to reversed phase liquid chromatography (RPLC) for subunit-level characterization of immuno-conjugates.
引用
收藏
页码：37 / 41
页数：5
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