Fluorescence Correlation Spectroscopy

被引：0
|
作者：
Beranova, Lenka [1 ]
Humpolickova, Jana [1 ]
Hof, Martin [1 ]
机构：
[1] Acad Sci Czech Republic, J Heyrovshy Inst Phys Chem, CR-18223 Prague 8, Czech Republic
来源：
CHEMICKE LISTY | 2009年 / 103卷 / 02期
关键词：
LIFETIME CORRELATION SPECTROSCOPY;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Basic principles of fluorescence correlation spectroscopy (FCS) are explained. The method affords diffusion coefficients and concentrations of fluorescent-labeled species by studying temporary fluctuations of fluorescence signal caused by diffusion through the focal volume of confocal microscope. In dual-color FCS, cross-correlation of two signals from molecules labeled with two spectrally shifted dyes allows to observe interactions between the two molecules. It is also possible to distinguish between the signals of two dyes with different lifetimes. FCS uses pulsed laser excitation.
引用
收藏
页码：125 / 129
页数：5
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