Localization and Expression of TREK-1, TREK-2, and TRAAK Channels in Rat Medial Vestibular Nuclei

被引：0

作者：

Kang, Dawon ^{[1
,2
]}

Cho, Young-Woo ^{[1
,2
]}

Kim, Wan Seung ^{[1
,2
]}

Han, Jaehee ^{[1
,2
]}

Jeon, Sea-Yuong ^{[2
,3
]}

Hur, Dong Gu ^{[2
,3
]}

Park, Jung Je ^{[2
,3
]}

Ahn, Seong-Ki ^{[2
,3
]}

机构：

[1] Gyeongsang Natl Univ, Dept Physiol, Sch Med, Jinju 660702, South Korea

[2] Gyeongsang Natl Univ, Inst Hlth Sci, Sch Med, Jinju 660702, South Korea

[3] Gyeongsang Natl Univ, Dept Otolaryngol, Sch Med, Jinju 660702, South Korea

来源：

JOURNAL OF INTERNATIONAL ADVANCED OTOLOGY | 2013年 / 9卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

POTASSIUM CHANNELS; K+ CHANNELS; MIGRAINE; STIMULATION; COLOCALIZATION; VERTIGO;

D O I：

暂无

中图分类号：

R76 [耳鼻咽喉科学];

学科分类号：

100213 ;

摘要：

Objective: The aim of this study was to examine the localization and expression of TREK-1, TREK-2 and TRAAK channels in rat medial vestibular nuclei by immunohistochemical staining, reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis. Materials and Methods: Twenty eight male Sprague-Dawley rats were used in this study. Antibodies for TREK-1, TREK-2 and TRAAK channels were used. Tissues containing medial vestibular nuclei were selectively for RT-PCR and Western blot analysis. Results: TREK-1, TREK-2 and TRAAK channels immunopositive neurons were distributed throughout the medial vestibular nuclei. RT-PCR data showed TREKs/TRAAK mRNAs were expressed in the medial vestibular nuclei. Western blot results also showed TREKs/TRAAK protein expression in the medial vestibular nuclei. Conclusion: This study may provide additional speculation into the role of K2p channels in the pathway between migraine and vestibular system.

引用

页码：75 / 81

页数：7

共 24 条

[21] Lipid and mechano-gated 2P domain K+ channels
Patel, AJ
Lazdunski, M
Honoré, E
[J]. CURRENT OPINION IN CELL BIOLOGY, 2001, 13 (04) : 422 - 427
[22] CNS distribution of members of the two-pore-domain (KCNK) potassium channel family
Talley, EM
Solórzano, G
Lei, QB
Kim, D
Bayliss, DA
[J]. JOURNAL OF NEUROSCIENCE, 2001, 21 (19) : 7491 - 7505
[23] Watson C., 1988, RAT BRAIN STEREOTAXI
[24] Immunohistochemical colocalization of TREK-1, TREK-2 and TRAAK with TRP channels in the trigeminal ganglion cells
Yamamoto, Yoshio
Hatakeyama, Taku
Taniguchi, Kazuyuki
[J]. NEUROSCIENCE LETTERS, 2009, 454 (02) : 129 - 133

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