Removal of Reactive Orange 16 by Immobilized Coprinus plicatilis in the Batch Shaking Bioreactor

Coprinus plicatilis, white rot fungi, capable of removing Reactive Orange 16 (Remazol Brillant Orange 3R) was immobilized in calcium alginate gel beads and used for decolorization of RO16 in aqueous in the Batch Shaking Bioreactor. The optimum conditions for immobilization of the microorganism, such as alginate and calcium ion concentration, initial cell amout, hardening time and bead size, were determined with a view to improving the RO16 removal rate. The characteristics of RO16 decolorization by immobilized fungal cells were investigated. The repeated use of immobilized cells for RO16 decolorization was performed and the results revealed that the bioactivity of immobilized cells was stable over 120 hours in the repeated batch cultivation RO16 removal. At the end of decolorization process, the metabolites of the dye decolorization produced by immobilized C. plicatilis, were analyzed via GC-MS. It was concluded that the removal of the dye by immobilized C. plicatilis was achieved.