Docosahexaenoic acid (DHA) induces apoptosis in human hepatocellular carcinoma cells

被引：0
作者：
Sun, Si-Nan [1 ]
Jia, Wei-Dong [1 ]
Chen, Hao [1 ]
Ma, Jin-Liang [1 ]
Ge, Yong-Sheng [1 ]
Yu, Ji-Hai [1 ]
Li, Jian-Sheng [1 ]
机构：
[1] Anhui Med Univ, Anhui Prov Hosp, Anhui Prov Key Lab Hepatopancreatobiliary Surg, Dept Gen Surg, Hefei 230001, Anhui, Peoples R China
来源：
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY | 2013年 / 6卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Docosahexaenoic (DHA); Bcl-2; caspase-3; MMP-9; POLYUNSATURATED FATTY-ACIDS; CANCER CELLS; GRANZYME-B; METABOLISM; EXPRESSION; INVASION; GROWTH; RATIO; LINE; DIET;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The docosahexaenoic (DHA), a omega-3 fatty acid, could play a beneficial inhibition of the incidence and progress of a series of human diseases including cancer. It has been report that DHA is involved in cell apoptosis. Recent studies show that the signal transduction pathway links with bcl-2, bax, caspase-3 and MMP-9 molecules. Therefore, we tested the relationship between DHA and cell apoptosis in human hepatocellular carcinoma cells (Bel-7402 cells). We show here that DHA induces Bel-7402 cells apoptosis after pre-treating cells with DHA. DHA down-regulates the protein expression of Bcl-2 and Bim mRNA level, and up-regulates caspase-3 activity and Bax expression level. We also found that DHA inhibits Bel-7402 cells migration. Basic on our studies, DHA may play a role in tumor invasion and survival.
引用
收藏
页码：281 / 289
页数：9
相关论文
共 26 条
  • [1] Docosahexaenoic acid intake decreases proliferation, increases apoptosis and decreases the invasive potential of the human breast carcinoma cell line MDA-MB-231
    Blanckaert, Vincent
    Ulmann, Lionel
    Mimouni, Virginie
    Antol, Johann
    Brancquart, Lucile
    Chenais, Benoit
    [J]. INTERNATIONAL JOURNAL OF ONCOLOGY, 2010, 36 (03) : 737 - 742
  • [2] Docosahexaenoic acid from a cultured microalga inhibits cell growth and induces apoptosis by upregulating Bax/Bcl-2 ratio in human breast carcinoma MCF-7 cells
    Chiu, LCM
    Wong, EYL
    Ooi, VEC
    [J]. SIGNAL TRANSDUCTION PATHWAYS, CHROMATIN STRUCTURE, AND GENE EXPRESSION MECHANISMS AS THERAPEUTIC TARGETS, 2004, 1030 : 361 - 368
  • [3] Docosahexaenoic acid inhibits invasion of human RT112 urinary bladder and PT45 pancreatic carcinoma cells via down-modulation of granzyme B expression
    D'Eliseo, Donatella
    Manzi, Laura
    Merendino, Nicolo
    Velotti, Francesca
    [J]. JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2012, 23 (05) : 452 - 457
  • [4] Granzyme B is expressed in urothelial carcinoma and promotes cancer cell invasion
    D'Eliseo, Donatella
    Pisu, Paola
    Romano, Chiara
    Tubaro, Andrea
    De Nunzio, Cosimo
    Morrone, Stefania
    Santoni, Angela
    Stoppacciaro, Antonella
    Velotti, Francesca
    [J]. INTERNATIONAL JOURNAL OF CANCER, 2010, 127 (06) : 1283 - 1294
  • [5] Role of ω-3 polyunsaturated fatty acids on cyclooxygenase-2 metabolism in brain-metastatic melanoma
    Denkins, Y
    Kempf, D
    Ferniz, M
    Nileshwar, S
    Marchetti, D
    [J]. JOURNAL OF LIPID RESEARCH, 2005, 46 (06) : 1278 - 1284
  • [6] DHA induces apoptosis by altering the expression and cellular location of GRP78 in colon cancer cell lines
    Fasano, Elena
    Serini, Simona
    Piccioni, Elisabetta
    Toesca, Amelia
    Monego, Giovanni
    Cittadini, Achille R.
    Ranelletti, Franco O.
    Calviello, Gabriella
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 2012, 1822 (11): : 1762 - 1772
  • [7] Regulation of Colorectal Cancer Cell Apoptosis by the n-3 Polyunsaturated Fatty Acids Docosahexaenoic and Eicosapentaenoic
    Giros, Anna
    Grzybowski, Mike
    Sohn, Vanessa R.
    Pons, Elisenda
    Fernandez-Morales, Jessica
    Xicola, Rosa M.
    Sethi, Puja
    Grzybowski, Jessica
    Goel, Ajay
    Boland, C. Richard
    Gassull, Miquel A.
    Llor, Xavier
    [J]. CANCER PREVENTION RESEARCH, 2009, 2 (08) : 732 - 742
  • [8] Diclofenac induces apoptosis in hepatocytes by alteration of mitochondrial function and generation of ROS
    Gómez-Lechón, MJ
    Ponsoda, X
    O'Connor, E
    Donato, T
    Castell, JV
    Jover, R
    [J]. BIOCHEMICAL PHARMACOLOGY, 2003, 66 (11) : 2155 - 2167
  • [9] Effects of a fish oil-based emulsion on rat hepatoma cell invasion in culture
    Hagi, Akifumi
    Nakayama, Mitsuo
    Miura, Yutaka
    Yagasaki, Kazumi
    [J]. NUTRITION, 2007, 23 (11-12) : 871 - 877
  • [10] Does diet affect breast cancer risk?
    Holmes, MD
    Willett, WC
    [J]. BREAST CANCER RESEARCH, 2004, 6 (04) : 170 - 178
123