Solution conformations of two naturally occurring RNA nucleosides: 3-Methyluridine and 3-methylpseudouridine

被引：13

作者：

Desaulniers, JP ^{[1
]}

Chui, HMP ^{[1
]}

Chow, CS ^{[1
]}

机构：

[1] Wayne State Univ, Dept Chem, Detroit, MI 48202 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2005年 / 13卷 / 24期

关键词：

3-methylpseudouridine; conformation; methylation;

D O I：

10.1016/j.bmc.2005.07.061

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The conformations of 3-methyluridine and 3-methylpseudouridine are determined using a combination of sugar proton coupling constants from 1D NMR spectra and 1D NOE difference spectroscopy. Both C-2'-endo and C-3'-endo conformations are observed for 3-methyluridine (59:41 37 degrees C, D2O) and 3-methylpseudouridine (51:49, 37 degrees C, D2O). 3-Methyluridine preferentially adopts an anti conformation in solution, whereas 3-methylpseudouridine is primarily in a syn conformation. anti/syn-Relationships are deduced by ID NOE difference spectroscopy. (c) 2005 Elsevier Ltd. All rights reserved.

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页码：6777 / 6781

页数：5

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