The piezomagnetic properties and negative piezomagnetic effect in Fe73.5Cu1Nb3Si13.5B9 amorphous alloy films were investigated. The results show that the Fe73.5Cu1Nb3Si13.5B9 amorphous alloy film has a significant positive piezomagnetic property in its quenched state. Furthermore, the inductance of the alloy film decreases substantially in the 0 similar to 0.66 kPa pressure range, whereas the compressive stress, sigma, increases. When sigma=0.66 kPa, the piezomagnetic effect value, S-1, reaches 5.5%. The ambient temperature has a significant influence on the sensitivity of the piezomagnetic property. The films show the optimal piezomagnetic properties and sensitivity stability in the temperature range of 20 similar to 30 degrees C. Increase in ambient temperature leads to decrease in the piezomagnetic property and sensitivity values, and the inner stress state of the film changes after annealing treatment. At an annealing temperature of 350 degrees C, the piezomagnetic effect of the film changes from "positive" to "negative". Furthermore, as the internal stress decreases, the value of vertical bar S-1 vertical bar decreases, and the piezomagnetic property of the film becomes less sensitive to the change in ambient temperature. At an annealing temperature of 555 degrees C, the value of vertical bar S-1 vertical bar is 0.59% (sigma=0.44 kPa). When the annealing temperature reaches the crystallization temperature, the piezomagnetic property of the film becomes less sensitive to the changes in ambient temperature.