Arabidopsis G-protein β subunit AGB1 interacts with NPH3 and is involved in phototropism

被引：14

作者：

Kansup, Jeeraporn ^{[1
]}

Tsugama, Daisuke ^{[1
,2
]}

Liu, Shenkui ^{[3
]}

Takano, Tetsuo ^{[1
]}

机构：

[1] Univ Tokyo, Asian Nat Environm Sci Ctr, Nishikyo Ku, Tokyo 1880002, Japan

[2] Univ Tokyo, Grad Sch Agr & Life Sci, Lab Plant Mol Genet, Bunkyo Ku, Tokyo 1138657, Japan

[3] Northeast Forestry Univ, Alkali Soil Nat Environm Sci Ctr, Harbin 150040, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2014年 / 445卷 / 01期

关键词：

Arabidopsis; Heterotrimeric G protein beta subunit; Nonphototropic hypocotyl 3; Protein-protein interaction; Phototropism; HETEROTRIMERIC G-PROTEIN; AUXIN TRANSPORT; EFFECTOR; LEAF;

D O I：

10.1016/j.bbrc.2014.01.106

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Heterotrimeric G proteins (G alpha, G beta and G gamma) have pleiotropic roles in plants, but molecular mechanisms underlying them remain to be elucidated. Here we show that Arabidopsis G beta (AGB1) interacts with NPH3, a regulator of phototropism. Yeast two-hybrid assays, in vitro pull-down assays and bimolecular fluorescence complementation assays showed that AGB1 and NPH3 physically interact. NPH3-null mutation (nph3) is known to completely abolish hypocotyl phototropism. Loss-of-function mutants of AGB1 (agb1-1 and agb1-2) showed decreased hypocotyl phototropism, and agb1/mph3 double mutants showed no hypocotyl phototropism. These results suggest that AGB1 is involved in the NPH3-mediated regulation of phototropism. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：54 / 57

页数：4

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