A new nano-optical sensor thin film cadmium sulfide doped in sol-gel matrix for assessment of α-amylase activity in human saliva

被引：41
作者：
Attia, M. S. [1 ]
Zoulghena, H. [1 ]
Abdel-Mottaleb, M. S. A. [1 ]
机构：
[1] Ain Shams Univ, Fac Sci, Dept Chem, Nanophotochem & Solarchem Lab, Cairo, Egypt
来源：
ANALYST | 2014年 / 139卷 / 04期
关键词：
THERMOSTABLE GLUCOAMYLASE; CDS; NANOPARTICLES; PURIFICATION; SINGLE; ASSAY;
D O I：
10.1039/c3an01645e
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A novel, simple, sensitive and precise spectrofluorimetric method is developed for measuring the activity of the alpha-amylase enzyme in human saliva. The remarkable quenching of the luminescence intensity at 634 nm of nano CdS doped in a sol-gel matrix by various concentrations of maltose (produced from the reaction of the enzyme with the starch substrate) was successfully used as an optical sensor for the assessment of alpha-amylase activity. The calibration plot was achieved over the concentration range 4.8 x 10(-10) to 1.2 x 10(-5) mol L-1 maltose with a correlation coefficient of 0.999 and a detection limit of 5.7 x 10(-11) mol L-1. The method was used satisfactorily for assessment of the a-amylase activity in a number of human saliva samples collected from various healthy volunteers.
引用
收藏
页码：793 / 800
页数：8
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