Study of the reaction between nucleic acids and Tb-BPMPHD-CTMAB complex and its analytical application

被引：12

作者：

Wu, X ^{[1
]}

Yang, JH ^{[1
]}

Huang, F ^{[1
]}

Wang, M ^{[1
]}

Sun, LM ^{[1
]}

Xu, GY ^{[1
]}

机构：

[1] Shandong Univ, Coll Chem, Lab Colloid & Interface Chem Educ Minist, Jinan 250100, Peoples R China

来源：

ANALYTICAL LETTERS | 1999年 / 32卷 / 12期

关键词：

nucleic acids; fluorescence quenching; Tb-BPMPHD-CTMAB complex;

D O I：

10.1080/00032719908542978

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The fluorescence quenching of the Tb-BPMPHD-CTMAB by nucleic acids is reported. The maximum fluorescence quenching was obtained at pH5.5, with maximum excitation and emission wavelengths at 300 and 543nm, respectively. Under optimal conditions, the differential value of fluorescence intensity between the absence and presence of nucleic acids was proportional to the concentration of nucleic acids over the range of 4.0x10(-8) ~ 1.0x10(-5) g/ml for fsDNA and 2.5x10(-8) ~ 6.15x10(-6) g/ml for yRNA, respectively. The corresponding detection limits are 9.0ng/ml for fsDNA and 5.0ng/ml for yRNA. The method was applied to the determination of DNA (or RNA) in synthetic samples. The results obtained were satisfactory.

引用

页码：2417 / 2425

页数：9

共 11 条

[1] ON LUMINESCENCE PROPERTIES OF SOME PURINES AND PYRIMIDINES - A STUDY BY FLUORESCENCE SPECTROPHOTOMETRY OF SITES OF PROTONATION AND OF TYPES OF LOWEST EXCITED SINGLET STATES [J].

BORRESEN, HC .

ACTA CHEMICA SCANDINAVICA, 1963, 17 (04) :921-&

[2] FLUORESCENCE REACTION OF TERBIUM(III) WITH NUCLEIC-ACIDS IN THE PRESENCE OF PHENANTHROLINE [J].

CI, YX ;

LI, YZ ;

CHANG, WB .

ANALYTICA CHIMICA ACTA, 1991, 248 (02) :589-594

[3] SELECTIVE DETERMINATION OF DNA BY ITS ENHANCEMENT EFFECT ON THE FLUORESCENCE EU3+-TETRACYCLINE COMPLEX [J].

CI, YX ;

LI, YZ ;

LIU, XJ .

ANALYTICAL CHEMISTRY, 1995, 67 (11) :1785-1788

[4] Fluorescence investigation of the interaction of safranine T with DNA [J].

Guo, XQ ;

Li, F ;

Zhao, YB .

ANALYTICAL LETTERS, 1998, 31 (06) :991-1005

[5]

Huang CZ, 1997, ANAL LETT, V30, P1305

[6]

HUANG CZ, 1997, ANAL LETT, V30, P867

[7] SIMPLE, RAPID, AND SENSITIVE DNA ASSAY PROCEDURE [J].

LABARCA, C ;

PAIGEN, K .

ANALYTICAL BIOCHEMISTRY, 1980, 102 (02) :344-352

[8] A NEW FLUOROMETRIC METHOD FOR RNA AND DNA DETERMINATION [J].

LEPECQ, JB ;

PAOLETTI, C .

ANALYTICAL BIOCHEMISTRY, 1966, 17 (01) :100-+

[9] Application of vitamin K-3 as a photochemical fluorescence probe in the determination of nucleic acids [J].

Li, WY ;

Xu, JG ;

Guo, XQ ;

Zhu, QZ ;

Zhao, YB .

ANALYTICAL LETTERS, 1997, 30 (02) :245-257

[10] FLUORESCENCE CHARACTERISTICS OF PURINES, PYRIMIDINES, AND THEIR DERIVATIVES - MEASUREMENT OF GUANINE IN NUCLEIC ACID HYDROLYZATES [J].

UDENFRIEND, S ;

ZALTZMAN, P .

ANALYTICAL BIOCHEMISTRY, 1962, 3 (01) :49-&

← 1 2 →