Determination of Toxic Activities in Bothrops spp. Snake Venoms Using Animal-Free Approaches: Correlation Between In Vitro Versus In Vivo Assays

被引：21

作者：

de Souza, Leticia Lopes ^{[1
]}

Stransky, Stephanie ^{[1
]}

Guerra-Duarte, Clara ^{[1
]}

Flor-Sa, Ana ^{[1
]}

Schneider, Francisco Santos ^{[1
]}

Kalapothakis, Evanguedes ^{[2
]}

Chavez-Olortegui, Carlos ^{[1
]}

机构：

[1] Univ Fed Minas Gerais, Dept Bioquim & Imunol, BR-31270901 Belo Horizonte, MG, Brazil

[2] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Biol Geral, BR-31270901 Belo Horizonte, MG, Brazil

来源：

TOXICOLOGICAL SCIENCES | 2015年 / 147卷 / 02期

关键词：

Bothrops venoms; in vitro assays; cytotoxic activity; lethal activity; hyaluronidase activity; AMINO-ACID OXIDASE; HEMORRHAGIC ACTIVITY; METALLOPROTEINASES; MECHANISMS; HYALURONIDASE; INSIGHTS; DAMAGE;

D O I：

10.1093/toxsci/kfv140

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

The main purpose of this study is to investigate the in vitro toxic effects of 5 Bothrops spp. snake venoms, which are part of the antigenic mixture used for the production of Brazilian antivenom, and evaluate their correlation with the in vivo toxic activities of Bothrops spp. venoms. The correlation analysis could be helpful for the replacement of living animals experimentation for in vitro bioassay. Cytotoxicity, L-amino acid oxidase (LAAO), proteolitic (serine and metalloproteinase), hyaluronidase (Hyal), and phospholipase A(2) (PLA(2)) activities were estimated and the correlation coefficient was determined for each activity in relation to lethality, edema, hemorrhage and necrosis induced in live animals by B. jararaca, B. alternatus, B. jararacussu, B. neuwiedi, and B. moojeni venoms. The lethal activity in mice was highly related to Hyal activity (r = 0.94, p < .05), edema related to PLA(2) activity (r = 0.94, p < .05), whereas the necrotizing activity showed high correlation with LAAO activity (r = 0.83, p < .05). A very significant correlation between in vitro cytotoxicity and LAAO activities was also observed (r = 0.97, p < .05).

引用

页码：458 / 465

页数：8

共 38 条

[1] ACUTE KIDNEY INJURY CAUSED BY Crotalus AND Bothrops SNAKE VENOM: A REVIEW OF EPIDEMIOLOGY, CLINICAL MANIFESTATIONS AND TREATMENT [J].

Albuquerque, Polianna L. M. M. ;

Jacinto, Camilla N. ;

Silva Junior, Geraldo B. ;

Lima, Juliana B. ;

Veras, Maria do Socorro B. ;

Daher, Elizabeth F. .

REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO, 2013, 55 (05) :295-301

[2] Cell cycle arrest evidence, parasiticidal and bactericidal properties induced by L-amino acid oxidase from Bothrops atrox snake venom [J].

Alves Paiva, Raquel de Melo ;

Figueiredo, Raquel de Freitas ;

Antonucci, Gilmara Ausech ;

Paiva, Helder Henrique ;

Pires Bianchi, Maria de Lourdes ;

Rodrigues, Kelly C. ;

Lucarini, Rodrigo ;

Caetano, Renato Cesar ;

Linhari Rodrigues Pietro, Rosemeire Cristina ;

Comes Martins, Carlos Henrique ;

de Albuquerque, Sergio ;

Sampaio, Suely Vilela .

BIOCHIMIE, 2011, 93 (05) :941-947

[3] Mechanisms of cell death induction by L-amino acid oxidase, a major component of ophidian venom [J].

Ande, Sudharsana Rao ;

Kommoju, Phaneeswara Rao ;

Draxl, Sigrid ;

Murkovic, Michael ;

Macheroux, Peter ;

Ghisla, Sandro ;

Ferrando-May, Elisa .

APOPTOSIS, 2006, 11 (08) :1439-1451

[4] Mechanisms of Vascular Damage by Hemorrhagic Snake Venom Metalloproteinases: Tissue Distribution and In Situ Hydrolysis [J].

Baldo, Cristiani ;

Jamora, Colin ;

Yamanouye, Norma ;

Zorn, Telma M. ;

Moura-da-Silva, Ana M. .

PLOS NEGLECTED TROPICAL DISEASES, 2010, 4 (06)

[5] Novel in vitro assays for assessing the haemorrhagic activity of snake venoms and for demonstration of venom metalloproteinase inhibitors [J].

Bee, A ;

Theakston, RDG ;

Harrison, RA ;

Carter, SD .

TOXICON, 2001, 39 (09) :1429-1434

[6] Toxicity of phospholipases A2 D49 (6-1 and 6-2) and K49 (Bj-VII) from Bothrops jararacussu venom [J].

Bonfim, V. L. ;

de Carvalho, D. D. ;

Ponce-Soto, L. A. ;

Kassab, B. H. ;

Marangoni, S. .

CELL BIOLOGY AND TOXICOLOGY, 2009, 25 (06) :523-532

[7] Isolation and biochemical, functional and structural characterization of a novel L-amino acid oxidase from Lachesis muta snake venom [J].

Bregge-Silva, Cristiane ;

Nonato, Maria Cristina ;

de Albuquerque, Sergio ;

Ho, Paulo Lee ;

Junqueira de Azevedo, Inacio L. M. ;

Vasconcelos Diniz, Marcelo Ribeiro ;

Lomonte, Bruno ;

Rucavado, Alexandra ;

Diaz, Cecilia ;

Maria Gutierrez, Jose ;

Arantes, Eliane Candiani .

TOXICON, 2012, 60 (07) :1263-1276

[8]

Calvete JJ, 2011, EXPERT REV PROTEOMIC, V8, P739, DOI [10.1586/EPR.11.61, 10.1586/epr.11.61]

[9] Antivenomics and venom phenotyping: A marriage of convenience to address the performance and range of clinical use of antivenoms [J].

Calvete, Juan J. .

TOXICON, 2010, 56 (07) :1284-1291

[10] Pharmacological characterization and neutralization of the venoms used in the production of Bothropic antivenom in Brazil [J].

Camey, KU ;

Velarde, DT ;

Sanchez, EF .

TOXICON, 2002, 40 (05) :501-509

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