Isolation and characterization of polymorphic microsatellite markers in the fish Garra orientalis (oriental sucking barb)

被引:4
作者
Su, Li-Wei [1 ]
Liu, Zhi-Zhi [1 ]
Wang, Cong-Tao [1 ]
Zeng, Zhen [1 ]
Liu, Ang-Yu [2 ]
Tang, Wen-Qiao [1 ]
Yang, Jin-Quan [1 ]
机构
[1] Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
[2] Natl Taiwan Univ, Coll Life Sci, Inst Zool, Taipei 106, Taiwan
基金
中国国家自然科学基金;
关键词
Microsatellite markers; Garra orientalis; Conservation; Genetic diversity; RIVER;
D O I
10.1007/s12686-012-9776-5
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
14 Polymorphic microsatellite markers were developed in the Garra orientalis. These new markers were tested in 23 individuals collected from the Wanquan River in the Hainan Island. The number of alleles (N-A), polymorphism information content, observed and expected heterozygosity per locus ranged from 8 to 25, 0.6788 to 0.9342, 0.5652 to 1.0000 and 0.7179 to 0.9585, respectively. Four loci are deviated from Hardy-Weinberg equilibrium due to the heterozygote deficiency. These markers can be used to study genetic diversity, population structure, conservation and rational utilization of this species.
引用
收藏
页码:231 / 233
页数:3
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