Nucleotide sequence, heterologous expression and novel purification of DNA ligase from Bacillus stearothermophilus

被引：8

作者：

Brannigan, JA

Ashford, SR

Doherty, AJ

Timson, DJ

Wigley, DB

机构：

[1] Univ Oxford, Sir William Dunn Sch Pathol, Oxford OX1 3RE, England

[2] Univ York, Dept Chem, York YO1 5DD, N Yorkshire, England

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1999年 / 1432卷 / 02期

基金：

英国惠康基金;

关键词：

DNA replication; DNA ligation; thermostable protein; NAD(+); Bacillus stearothermophilus;

D O I：

10.1016/S0167-4838(99)00122-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene for DNA ligase (EC 6.5.1.2) from thermophilic bacterium Bacillus stearothermophilus NCA1503 has been cloned and the complete nucleotide sequence determined. The ligase gene encodes a protein 670 amino acids in length. The gene was overexpressed in Escherichia coli and the enzyme has been purified to homogeneity. Preliminary characterisation confirms that it is a thermostable, NAD(+)-dependent DNA ligase. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：413 / 418

页数：6

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