Preparation and evaluation of Vibrio cholerae O1 EL Tor Ogawa lipopolysaccharide-tetanus toxoid conjugates

被引：2

作者：

Cabrera, O ^{[1
]}

Martínez, ME ^{[1
]}

Cuello, M ^{[1
]}

Soto, CR ^{[1
]}

Valmaseda, T ^{[1
]}

Cedré, B ^{[1
]}

González, GS ^{[1
]}

机构：

[1] Finlay Inst, Dept Immunol, Havana, Cuba

来源：

VACCINE | 2006年 / 24卷

关键词：

LM detoxification; Vibrio cholerae; conjugate vaccine;

D O I：

10.1016/j.vaccine.2005.01.130

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The lipopolysaccharide (LPS) of Vibrio cholerae is considered one of the most important antigens from the point of view of immunogenicity in these bacteria. We have undertaken detoxification of this LPS by basic hydrolysis and the resultant amine groups were used for their conjugation to tetanus toxoid as carrier protein using carbodiimide-mediated coupling. The resulting conjugates were inoculated in Balb/c mice for immunogenicity studies. The anti-LPS IgG and vibriocidal antibodies were measured in serum. The antigenicity of this conjugated was evaluated by ELISA, with serums of humans vaccinated with a strain genetically modified. The conjugated elicited: high titers of IgG anti-LPS, high titers of vibriocidal antibodies and there was recognition of LPS by antibodies from cholerae immunised human serum. These results show that the conjugated LPS obtained by us, could be evaluated like a potential vaccine for human use. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：S74 / S75

页数：2

共 7 条

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