Genistein Inhibits ox-LDL-induced VCAM-1, ICAM-1 and MCP-1 Expression of HUVECs Through Heme Oxygenase-1

被引:60
作者
Zhang, Hua-ping [1 ]
Zheng, Feng-li [1 ]
Zhao, Jia-hui [1 ]
Guo, Dong-xing [2 ]
Chen, Xiao-long [3 ]
机构
[1] Shanxi Med Univ, Minist Educ, Key Lab, Taiyuan 030001, Peoples R China
[2] Shanxi Med Univ, Dept Pharmacol, Sch Basic Med Sci, Taiyuan 030001, Peoples R China
[3] Shanxi Med Univ, Dept Anat, Sch Basic Med Sci, Taiyuan 030001, Peoples R China
关键词
Genistein; Atherosclerosis; Inflammation; HO-1; Nrf2; CORONARY-ARTERY-DISEASE; ENDOTHELIAL-CELLS; CARDIOVASCULAR-DISEASE; THERAPEUTIC TARGETS; ADHESION MOLECULES; ATHEROSCLEROSIS; INFLAMMATION; INDUCTION; MICE; PROLIFERATION;
D O I
10.1016/j.arcmed.2012.12.001
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Background and Aims. Genistein, a principal component of soybean isoflavones, plays an important role in the prevention of atherosclerosis. However, the detailed mechanisms have not been fully investigated. The aims of this study were to evaluate the anti-atherosclerotic effect and investigate potential pharmacological mechanism of genistein. Methods. A model of oxidized low-density lipoprotein (ox-LDL)-induced injury in on human umbilical vein endothelial cells (HUVECs) was established to evaluate the protective role of genistein. Macrophage/monocyte chemoattractant protein-1 (MCP-1), vascular cellular adhesion molecule-1 (VCAM-1) and intracellular adhesion molecule-1 (ICAM-1) secretion and their messenger RNA transcription were observed via enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase PCR (RT-PCR). Meanwhile, the study investigated the role of Nrf2/HO-1 pathway during the process. Results. Pretreatment with genistein markedly reduced ox-LDL-induced MCP-1, VCAM-1 and ICAM-1 secretion and mRNA transcription, which was further decreased by the inducer of HO and reversed by the inhibitor of HO; additionally, the effects were accompanied with upregulating HO-1 mRNA and protein expression and markedly abolished with Nrf2 siRNA. Conclusions. Anti-inflammatory effect of genistein on endothelial cells may be associated with the activation of Nrf2/HO-1 pathway. (C) 2013 IMSS. Published by Elsevier Inc.
引用
收藏
页码:13 / 20
页数:8
相关论文
共 50 条
[41]   The Expression of Heme Oxygenase-1 Induced by Lansoprazole [J].
Takagi, Tomohisa ;
Naito, Yuji ;
Yoshikawa, Toshikazu .
JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION, 2009, 45 (01) :9-13
[42]   Spilanthol inhibits TNF-α-induced ICAM-1 expression and pro-inflammatory responses by inducing heme oxygenase-1 expression and suppressing pJNK in HaCaT keratinocytes [J].
Huang, Chun-Hsun ;
Chang, Li-Chun ;
Hu, Sindy ;
Hsiao, Chien-Yu ;
Wu, Shu-Ju .
MOLECULAR MEDICINE REPORTS, 2018, 18 (03) :2987-2994
[43]   IDENTICAL EXPRESSION OF ELAM-1, VCAM-1, AND ICAM-1 IN SARCOIDOSIS AND USUAL INTERSTITIAL PNEUMONITIS [J].
VANDINTHERJANSSEN, ACHM ;
VANMAARSSEVEEN, TCMT ;
ECKERT, H ;
NEWMAN, W ;
MEIJER, CJLM .
JOURNAL OF PATHOLOGY, 1993, 170 (02) :157-164
[44]   EXPRESSION OF ICAM-1 AND VCAM-1 AND MONOCYTE ADHERENCE IN ARTERIES EXPOSED TO ALTERED SHEAR-STRESS [J].
WALPOLA, PL ;
GOTLIEB, AI ;
CYBULSKY, MI ;
LANGILLE, BL .
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 1995, 15 (01) :2-10
[45]   Atractylenolide I restores HO-1 expression and inhibits Ox-LDL-induced VSMCs proliferation, migration and inflammatory responses in vitro [J].
Li, Weifeng ;
Zhi, Wenbing ;
Liu, Fang ;
He, Zehong ;
Wang, Xiuei ;
Niu, Xiaofeng .
EXPERIMENTAL CELL RESEARCH, 2017, 353 (01) :26-34
[46]   A allele of ICAM-1 rs5498 and VCAM-1 rs3181092 is correlated with increased risk for periodontal disease [J].
Sun, Qijun ;
Zhang, Zongxin ;
Ou, Yuejian .
OPEN LIFE SCIENCES, 2019, 14 (01) :638-646
[47]   THE EXPRESSION OF THE ADHESION MOLECULES ICAM-1, VCAM-1, PECAM, AND E-SELECTIN IN HUMAN ATHEROSCLEROSIS [J].
DAVIES, MJ ;
GORDON, JL ;
GEARING, AJH ;
PIGOTT, R ;
WOOLF, N ;
KATZ, D ;
KYRIAKOPOULOS, A .
JOURNAL OF PATHOLOGY, 1993, 171 (03) :223-229
[48]   Astaxanthin suppresses End MT by LOX-1 pathway in ox-LDL-induced HUVECs [J].
Zhu, Zhongsheng ;
Li, Jinyu ;
Tong, Rui ;
Zhang, Xiaorong ;
Yu, Bo .
EUROPEAN JOURNAL OF INFLAMMATION, 2022, 20
[49]   Structural requirements of flavonoids to induce heme oxygenase-1 expression [J].
Croft, K. D. ;
Zhang, D. ;
Jiang, R. ;
Ayer, A. ;
Shengule, S. ;
Payne, R. J. ;
Ward, N. C. ;
Stocker, R. .
FREE RADICAL BIOLOGY AND MEDICINE, 2017, 113 :165-175
[50]   Irradiation induced expression of CD31, ICAM-1 and VCAM-1 in human microvascular endothelial cells [J].
Quarmby, S ;
Hunter, RD ;
Kumar, S .
ANTICANCER RESEARCH, 2000, 20 (5B) :3375-3381