A lysosomal targeting fluorescent probe and its zinc imaging in SH-SY5Y human neuroblastoma cells

被引：23
作者：
Duan, Houna [1 ]
Ding, Yu [1 ]
Huang, Chusen [2 ,3 ]
Zhu, Weiping [1 ]
Wang, Rui [4 ]
Xu, Yufang [1 ]
机构：
[1] East China Univ Sci & Technol, State Key Lab Bioreactor Engineerring, Shanghai Key Lab Chem Biol, Sch Pharm, Shanghai 200237, Peoples R China
[2] Shanghai Normal Univ, Educ Minist, Key Lab Resource Chem, Shanghai 200234, Peoples R China
[3] Shanghai Normal Univ, Shanghai Key Lab Rare Earth Funct Mat, Dept Chem, Coll Life & Environm Sci, Shanghai 200234, Peoples R China
[4] East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China
来源：
CHINESE CHEMICAL LETTERS | 2019年 / 30卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Naphthalimide; Zinc ions; Cellular imaging; Lysosome; Fluorescent probe; OXIDATIVE STRESS; PROSTATE-CANCER; METAL-IONS; AUTOPHAGY; SENSORS; DESIGN; INDICATORS; MEMBRANE; RELEASE;
D O I：
10.1016/j.cclet.2018.03.016
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Zinc plays a significant role in oxidative balance and central nervous systems. Herein, we reported a highly sensitive fluorescent probe DR, bearing a morpholine group and a BPEN ligand in the naphthalimide fluorophore. Upon Zn2+ binding, DR exhibited remarkable fluorescence enhancement, and showed high sensitivity to Zn2+ with the association constant of 4.9 x 10(8) L/mol, and the detection limit of 15 nmol/L. Confocal imaging experiments indicated that DR was able to localize to lysosomes in MCF-7 cells. Moreover, upon H2O2 stimulation in SH-SY5Y cells, endogenous release of Zn2+ was observed. (C) 2018 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：55 / 57
页数：3
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