Interleukin-6 (IL-6) mediated the increased contraction of distal colon in streptozotocin-induced diabetes in rats via IL-6 receptor pathway

被引：0

作者：

Chang, Xin-Wen ^{[1
]}

Qin, Ying ^{[2
]}

Jin, Zhi ^{[2
]}

Xi, Tao-Fang ^{[2
]}

Yang, Xiao ^{[2
]}

Lu, Ze-Hao ^{[2
]}

Tang, Yu-Ping ^{[1
]}

Cai, Wen-Ting ^{[2
]}

Chen, Shao-Jun ^{[2
]}

Xie, Dong-Ping ^{[2
]}

机构：

[1] Tongji Univ, Sch Med, Dept Clin & Translat Res Ctr, Shanghai Matern & Infant Hosp 1, Shanghai 200040, Peoples R China

[2] Tongji Univ, Sch Med, Dept Physiol, Shanghai Peoples Hosp 10, Shanghai 200092, Peoples R China

来源：

INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY | 2015年 / 8卷 / 05期

关键词：

Diabetes mellitus; colon motility; IL-6; receptor; CORONARY-HEART-DISEASE; EXPERIMENTAL COLITIS; SIGNAL TRANSDUCER; SMOOTH-MUSCLE; TNF-ALPHA; MOTILITY; INFLAMMATION; DYSFUNCTION; MICE; EXPRESSION;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Colonic dysmotility occurs in diabetes and blood plasma interleukin (IL)-6 levels are significantly elevated in type 1 diabetes mellitus. The aim of this study was to investigate whether IL-6 and the IL-6 receptor pathway mediates colonic dysfunction in type 1 diabetes mellitus. Male SD rats were treated with a single intraperitoneally injected dose of streptozotocin (STZ), and those displaying sustained high blood glucose were selected as diabetes mellitus models. Longitudinal muscle strips of colon were prepared to monitor colonic contraction in vitro. Contractile responses of strips of colon were recorded following treatment with IL-6 in control animals, and following anti IL-6 antibody treatment in STZ-induced diabetes in rats. Concentration of IL-6 in plasma and colon were determined by ELISA. Expressions of IL-6 a-receptor and IL-6 beta-receptor in colon tissues were determined by immunohistochemistry or Western blot analysis. The non-diabetes rats treated with IL-6 and the untreated diabetes rats showed increased contraction of distal colon, whereas the diabetes rats treated with anti-IL-6 antibody showed decreased contraction of distal colon compared with the untreated diabetes rats. The IL-6 levels of plasma but not colon increased in diabetes rats. The expression of IL-6 a-receptor increased in diabetes rats. These results indicate that diabetes rats show an increase in the contractions of distal colon partly via the IL-6-IL-6 receptor pathway.

引用

页码：4514 / 4524

页数：11

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