Microsatellite markers for the red band needle blight pathogen, Dothistroma septosporum

被引：31

作者：

Barnes, I. ^{[1
]}

Cortinas, M. N. ^{[1
]}

Wingfield, M. J. ^{[1
]}

Wingfield, B. D. ^{[1
]}

机构：

[1] Univ Pretoria, Dept Genet, Forestry & Agr Biotechnol Inst, ZA-0002 Pretoria, South Africa

来源：

MOLECULAR ECOLOGY RESOURCES | 2008年 / 8卷 / 05期

基金：

美国安德鲁·梅隆基金会; 新加坡国家研究基金会;

关键词：

cross-species amplification; fungi; haploid; Mycosphaerella pini; population genetics; sequence variation;

D O I：

10.1111/j.1755-0998.2008.02142.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Twelve microsatellite markers were developed for population analyses of the fungal pathogen, Dothistroma septosporum. Intersimple sequence repeat polymerase chain reaction (ISSR-PCR) and an enrichment protocol (fast isolation by amplified fragment length polymorphism of sequences containing repeats [FIASCO]) were both used to identify 28 unique microsatellite regions in the genome. From 22 primer pairs designed, 12 were polymorphic. These markers, screened on two populations representing 42 isolates, produced 40 alleles across all loci with an allelic diversity of 0.09-0.76 per locus. Cross-species amplification showed variable success with Dothistroma rhabdoclinis and Mycosphaerella dearnessi and some sequence variation within isolates of Dothistroma pini. These markers will be used to further study the population structure and diversity of D. septosporum.

引用

页码：1026 / 1029

页数：4

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