SNP identification and allelic-specific PCR markers development for TaGW2, a gene linked to wheat kernel weight

被引:117
作者
Yang, Zibo [1 ,2 ]
Bai, Zhiyuan [1 ,2 ]
Li, Xiaolin [1 ,2 ]
Wang, Pei [1 ,2 ]
Wu, Qingxia [1 ,2 ]
Yang, Lin [1 ,2 ]
Li, Liqun [1 ,2 ]
Li, Xuejun [1 ,2 ]
机构
[1] NW A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China
[2] NW A&F Univ, Coll Agron, Yangling 712100, Shaanxi, Peoples R China
关键词
TRITICUM-AESTIVUM L; RICE GRAIN WIDTH; BREAD WHEAT; NATURAL VARIATION; QTL ANALYSIS; MAJOR QTL; YIELD; SIZE; LOCI; DNA;
D O I
10.1007/s00122-012-1895-6
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
TaGW2, an orthologous gene of rice OsGW2, has been associated with kernel width and weight of bread wheat (Triticum aestivum). Difference in TaGW2 coding sequence was not found among different wheat varieties in previous researches. In this study, we found eight exons and seven introns in TaGW2 with a full-length cDNA sequence of 1,275 bp, which contains a conserved function domain and seven splice sites that shared homology with rice OsGW2. A single T-base insertion in the eighth exon of TaGW2 on chromosome 6A was detected in a large-kernel wheat variety, Lankaodali. This insertion mutation reduces the coding protein sequence from normal 424 amino acids (similar to 47.2 kDa) to 328 amino acids (similar to 37.1 kDa) by truncating 96 amino acids. The result was validated by identifying histidine-tagged TaGW2 proteins encoded by both alleles of the mutant and the wild types in SDS-PAGE. Allele-specific PCR markers were developed based on the single nucleotide polymorphism (SNP) site. The SNP markers were genotyped for an F-2 segregation population from the cross of Lankaodali x Chinese Spring. Seed traits of F-2:3 families were evaluated in three different environments. The association analysis indicated that F-2:3 families with the mutated TaGW2 allele significantly increased kernel width (KW) and thousand-kernel weight (TKW), and slightly improved kernel length (KL). Using the SNP markers, another two varieties harbored the mutated TaGW2 allele were successfully identified from 22 additional wheat varieties, and they both have large KW and TKW. Cloning and sequencing of the gene further confirmed the functions of the mutated allele of TaGW2 in the two large kernel varieties. The results suggested that TaGW2 may negatively regulate kernel size variation, which shares the same function as OsGW2 in rice. The successful development of SNP markers provides a useful tool for improving kernel yield in wheat.
引用
收藏
页码:1057 / 1068
页数:12
相关论文
共 51 条
[1]   HOMOEOLOGOUS RELATIONSHIPS OF RICE, WHEAT AND MAIZE CHROMOSOMES [J].
AHN, S ;
ANDERSON, JA ;
SORRELLS, ME ;
TANKSLEY, SD .
MOLECULAR & GENERAL GENETICS, 1993, 241 (5-6) :483-490
[2]  
Arumuganathan K., 1991, Plant Mol Biol Rep, V9, P208, DOI [10.1007/BF02672069, DOI 10.1007/BF02672069]
[3]   Mapping of quantitative trait loci determining agronomic important characters in hexaploid wheat (Triticum aestivum L.) [J].
Börner, A ;
Schumann, E ;
Fürste, A ;
Cöster, H ;
Leithold, B ;
Röder, MS ;
Weber, WE .
THEORETICAL AND APPLIED GENETICS, 2002, 105 (6-7) :921-936
[4]   REGULATORY ELEMENTS IN THE 1ST INTRON CONTRIBUTE TO TRANSCRIPTIONAL CONTROL OF THE HUMAN ALPHA-1(I) COLLAGEN GENE [J].
BORNSTEIN, P ;
MCKAY, J ;
MORISHIMA, JK ;
DEVARAYALU, S ;
GELINAS, RE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (24) :8869-8873
[5]   Association mapping of kernel size and milling quality in wheat (Triticum aestivum L.) cultivars [J].
Breseghello, F ;
Sorrells, ME .
GENETICS, 2006, 172 (02) :1165-1177
[6]   A rapid DNA minipreparation method suitable for AFLP and other PCR applications [J].
Chen, DH ;
Ronald, PC .
PLANT MOLECULAR BIOLOGY REPORTER, 1999, 17 (01) :53-57
[7]   Conserved intron positions in ancient protein modules [J].
de Roos, Albert D. G. .
BIOLOGY DIRECT, 2007, 2 (1)
[8]   Molecular marker analysis of kernel size and shape in bread wheat [J].
Dholakia, BB ;
Ammiraju, JSS ;
Singh, H ;
Lagu, MD ;
Röder, MS ;
Rao, VS ;
Dhaliwal, HS ;
Ranjekar, PK ;
Gupta, VS .
PLANT BREEDING, 2003, 122 (05) :392-395
[9]   Microcolinearity between a 2-cM region encompassing the grain protein content locus Gpc-6B1 on wheat chromosome 6B and a 350-kb region on rice chromosome [J].
Distelfeld, Assaf ;
Uauy, Cristobal ;
Olmos, Sofia ;
Schlatter, Ana R. ;
Dubcovsky, Jorge ;
Fahima, Tzion .
FUNCTIONAL & INTEGRATIVE GENOMICS, 2004, 4 (01) :59-66
[10]   GS3, a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative transmembrane protein [J].
Fan, CH ;
Xing, YZ ;
Mao, HL ;
Lu, TT ;
Han, B ;
Xu, CG ;
Li, XH ;
Zhang, QF .
THEORETICAL AND APPLIED GENETICS, 2006, 112 (06) :1164-1171