CircRNA CTNNB1 (circCTNNB1) ameliorates cerebral ischemia/reperfusion injury by sponging miR-96-5p to up-regulate scavenger receptor class B type 1 (SRB1) expression

被引:14
作者
Chen, Chun [1 ]
Chang, Xiaolong [1 ]
Zhang, Shifei [1 ]
Zhao, Qi [1 ]
Lei, Chunyan [1 ]
机构
[1] Kunming Med Univ, Dept Neurol, Affiliated Hosp 1, Kunming, Yunnan, Peoples R China
关键词
Cerebral ischemia-reperfusion injury; oxygen glucose deprivation and reperfusion; blood-brain barrier; circRNA CTNNB1; scavenger receptor class B type I; miR-96-5p; BLOOD-BRAIN-BARRIER; MICE; IDENTIFICATION; MODEL; SR-B1;
D O I
10.1080/21655979.2022.2061304
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Emerging studies show that circRNA catenin beta 1 (circCTNNB1) plays a critical role in cancer. However, the expression and function of circCTNNB1 in cerebral ischemia/reperfusion injury (IRI) have not been reported. The present study discovered that circCTNNB1 and scavenger receptor class B type 1 (SRB1) expression levels were significantly down-regulated in mouse astrocytes (mAS) treated with oxygen glucose deprivation and reperfusion (OGD/R), and similar results were observed in a mouse middle cerebral artery occlusion model. Overexpression of circCTNNB1 alleviated cell apoptosis, oxidative stress and the inflammatory response induced by OGD/R in vitro. Up-regulation of circCTNNB1 increased SRB1 expression levels to protect mAS cells from OGD/R-induced damage. CircCTNNB1 and SRB1 interacted with miR-96-5p, and the overexpression of miR-96-5p efficiently reversed the function of circCTNNB1 in OGD/R-treated mAS cells. CircCTNNB1 protected against cerebral ischemia-reperfusion injury by up-regulating SRB1 in vivo. In conclusion, our findings suggest that circCTNNB1 acts as a competitive endogenous RNA for miR-96-5p to alleviate cerebral IRI, which provides novel evidence that circCTNNB1 and SRB1 may be biomarkers and therapeutic targets for cerebral IRI.
引用
收藏
页码:10258 / 10273
页数:16
相关论文
共 49 条
[1]   Identification of scavenger receptor SR-BI as a high density lipoprotein receptor [J].
Acton, S ;
Rigotti, A ;
Landschulz, KT ;
Xu, SZ ;
Hobbs, HH ;
Krieger, M .
SCIENCE, 1996, 271 (5248) :518-520
[2]   Circular RNAs open a new chapter in cardiovascular biology [J].
Aufiero, Simona ;
Reckman, Yolan J. ;
Pinto, Yigal M. ;
Creemers, Esther E. .
NATURE REVIEWS CARDIOLOGY, 2019, 16 (08) :503-514
[3]  
Ben Haim Lucile, 2015, Front Cell Neurosci, V9, P278, DOI 10.3389/fncel.2015.00278
[4]   Circular RNA cZNF292 silence alleviates OGD/R-induced injury through up-regulation of miR-22 in rat neural stem cells (NSCs) (Publication with Expression of Concern. See vol. 50, pg. 223, 2022) [J].
Cao, Yaqin ;
Liu, Hui ;
Zhang, Jun ;
Dong, Yubin .
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, 2020, 48 (01) :594-601
[5]   Recombinant high-density lipoproteins and their use in cardiovascular diseases [J].
Cao, Yi-ni ;
Xu, Lu ;
Han, Ying-chun ;
Wang, Yu-nan ;
Liu, George ;
Qi, Rong .
DRUG DISCOVERY TODAY, 2017, 22 (01) :180-185
[6]   Identification and functional analysis of microRNAs in rats following focal cerebral ischemia injury [J].
Duan, Xianchun ;
Gan, Jianghua ;
Peng, Dai-Yin ;
Bao, Qiuyu ;
Xiao, Ling ;
Wei, Liangbing ;
Wu, Jian .
MOLECULAR MEDICINE REPORTS, 2019, 19 (05) :4175-4184
[7]   An integrated encyclopedia of DNA elements in the human genome [J].
Dunham, Ian ;
Kundaje, Anshul ;
Aldred, Shelley F. ;
Collins, Patrick J. ;
Davis, CarrieA. ;
Doyle, Francis ;
Epstein, Charles B. ;
Frietze, Seth ;
Harrow, Jennifer ;
Kaul, Rajinder ;
Khatun, Jainab ;
Lajoie, Bryan R. ;
Landt, Stephen G. ;
Lee, Bum-Kyu ;
Pauli, Florencia ;
Rosenbloom, Kate R. ;
Sabo, Peter ;
Safi, Alexias ;
Sanyal, Amartya ;
Shoresh, Noam ;
Simon, Jeremy M. ;
Song, Lingyun ;
Trinklein, Nathan D. ;
Altshuler, Robert C. ;
Birney, Ewan ;
Brown, James B. ;
Cheng, Chao ;
Djebali, Sarah ;
Dong, Xianjun ;
Dunham, Ian ;
Ernst, Jason ;
Furey, Terrence S. ;
Gerstein, Mark ;
Giardine, Belinda ;
Greven, Melissa ;
Hardison, Ross C. ;
Harris, Robert S. ;
Herrero, Javier ;
Hoffman, Michael M. ;
Iyer, Sowmya ;
Kellis, Manolis ;
Khatun, Jainab ;
Kheradpour, Pouya ;
Kundaje, Anshul ;
Lassmann, Timo ;
Li, Qunhua ;
Lin, Xinying ;
Marinov, Georgi K. ;
Merkel, Angelika ;
Mortazavi, Ali .
NATURE, 2012, 489 (7414) :57-74
[8]   Expanded identification and characterization of mammalian circular RNAs [J].
Guo, Junjie U. ;
Agarwal, Vikram ;
Guo, Huili ;
Bartel, David P. .
GENOME BIOLOGY, 2014, 15 (07)
[9]   Scavenger Receptor BI and High-Density Lipoprotein Regulate Thymocyte Apoptosis in Sepsis [J].
Guo, Ling ;
Zheng, Zhong ;
Ai, Junting ;
Howatt, Deborah A. ;
Mittelstadt, Paul R. ;
Thacker, Seth ;
Daugherty, Alan ;
Ashwell, Jonathan D. ;
Remaley, Alan T. ;
Li, Xiang-An .
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2014, 34 (05) :966-975
[10]   Astrocyte mediated modulation of blood-brain barrier permeability does not correlate with a loss of tight junction proteins from the cellular contacts [J].
Hamm, S ;
Dehouck, B ;
Kraus, J ;
Wolburg-Buchholz, K ;
Wolburg, H ;
Risau, W ;
Cecchelli, R ;
Engelhardt, B ;
Dehouck, MP .
CELL AND TISSUE RESEARCH, 2004, 315 (02) :157-166