Subcritical water extraction of bioactive compounds from Orostachys japonicus A. Berger (Crassulaceae)

被引:76
作者
Ko, Min-Jung [1 ]
Nam, Hwa-Hyun [2 ]
Chung, Myong-Soo [2 ]
机构
[1] Hankyong Natl Univ, Dept Food Sci & Biotechnol, Global K Food Res Ctr, Anseong 17579, South Korea
[2] Ewha Womans Univ, Dept Food Sci & Engn, Seoul 03760, South Korea
基金
新加坡国家研究基金会;
关键词
FLAVONOIDS STRUCTURE; ANTIOXIDANT POWER; MECHANISMS; CAPACITY; KINETICS; ABILITY; FRAP;
D O I
10.1038/s41598-020-67508-2
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Subcritical-water extraction is an ecofriendly method for extracting antioxidant compounds only using water. The Subcritical-water extraction was employed for the extraction of bioactive compounds from Orostachys japonicus known as rock pine by investigating the use of various temperatures (110-260 degrees C) and extraction times (5-20 min). The Subcritical-water extraction condition at 220 degrees C for 15 min; the total phenolics content (39.9 +/- 4.1 mg/g), flavonoids content (11.4 +/- 0.6 mg/g), and antioxidant activities (90.3 +/- 2.2%, 96.0 +/- 2.9%, and 662.4 +/- 17.2 mg/g) of Subcritical-water extract were higher under this condition than for extraction with either methanol or ethanol. Triterpene saponins were observed only in subcritical-water extraction condition at 220 degrees C for 15 min. Further, some of its phenolic constituents; gallic acid, quercetin, and kaempferol were quantified by high performance liquid chromatography. Subcritical-water extraction is an effective method for extracting valuable bioactive compounds from Orostachys japonicus.
引用
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页数:10
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