Karyotype analysis using FISH (fluorescence in situ hybridization) in Fragaria

被引：14

作者：

Rho, Il Rae ^{[2
,3
]}

Hwang, Yoon Jung ^{[1
]}

Lee, Hyung Il ^{[1
]}

Lee, Choon-Hwan ^{[3
]}

Lim, Ki Byung ^{[1
]}

机构：

[1] Kyungpook Natl Univ, Coll Agr & Life Sci, Sch Appl Biosci, Taegu 702701, South Korea

[2] Natl Inst Hort & Herbal Sci, Protected Hort Expt Stn, Pusan 618300, South Korea

[3] Pusan Natl Univ, Dept Mol Biol, Pusan 609735, South Korea

来源：

SCIENTIA HORTICULTURAE | 2012年 / 136卷

关键词：

Chromosome; FISH; Strawberry; 5S rDNA; 45S rDNA; RIBOSOMAL-RNA GENES; CHROMOSOMES; BEHAVIOR;

D O I：

10.1016/j.scienta.2011.12.025

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

The karyotypes of Fragaria species, diploid (Fragaria vesca 'UC-01', 'Hawaii-30', Fragaria nilgerrensis 'Whiteberry') and octoploid (Fragaria x ananassa 'Akihime'). were analyzed using a method based on fluorescence in situ hybridization (FISH), using ribosomal DNA probes (5S rDNA and 45S rDNA). All diploid species of F. vesca and F. nilgerrensis had 2n = 14 chromosomes, while F. x ananassa, an octoploid species, were 2n = 56. The total length of somatic metaphase chromosome was measured as 12.22, 13.61, and 11.19 mu m for 'UC-01', 'Hawaii-30', and 'Whiteberry', respectively, and 'Akihime' measured 94.23 mu m. The karyotypes were composed of 6 pairs of metacentric and 1 pair of submetacentric in 'UC-01' and 'Whiteberry', and 5 pairs of metacentric and 2 pairs of submetacentric in 'Hawaii-30'. 5S rDNA loci were detected in 1 pair from 'UC-01', 'Hawaii-30' and 'Whiteberry', and 45S rDNA loci were detected in 3 pairs from 'UC-01' and 'Hawaii-30', whereas 45S rDNA loci were detected in 2 pairs from 'Whiteberry'. In octoploid strawberry 'Akihime', 45S rDNA signals were observed at 12 chromosomes, and 5S rDNA signals were observed at 2 chromosomes. Crown Copyright (c) 2012 Published by Elsevier B.V. All rights reserved.

引用

页码：95 / 100

页数：6

共 29 条

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