Identification and Expression Profiling of a Low Oxygen Regulated Gene in Peach Rootstocks Using RT-PCR

被引:0
作者
Amador, M. L. [1 ]
Rubio-Cabetas, M. J. [1 ]
机构
[1] Ctr Invest & Tecnol Agroalimentaria Aragon CITA, Unidad Fruticultura, Zaragoza 50059, Spain
来源
VII INTERNATIONAL PEACH SYMPOSIUM | 2012年 / 962卷
关键词
abiotic stress; hypoxia; PDC; RACE-PCR; rootstocks; RT-PCR; PYRUVATE DECARBOXYLASE GENES; WATERLOGGING TOLERANCE; ARABIDOPSIS; PLANTS;
D O I
暂无
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The breeding strategy for adaptative traits is based upon the understanding of the physiological and molecular aspects involved in such adaptative response. Waterlogging is an abiotic stress resulting from complete soil inundation and creating a low oxygen (hypoxia) environment in the plants roots. The molecular basis for the adaptation of root tissues to low oxygen conditions in waterlogged soils remains largely unknown. Different studies have shown that fermentation pathways play essential roles for survival under these conditions. Alcoholic fermentation is the most important fermentative pathway for energy production. It is a relatively simple process, where pyruvate is converted into acetaldehyde by pyruvate decarboxylase (PDC), and acetaldehyde is subsequently converted into ethanol by alcohol dehydrogenase (ADH). PDC is the first enzyme channelling carbon towards the ethanolic fermentation pathway and is considered to be the rate-limiting step in this pathway. This suggests a key regulatory role for the first enzyme of ethanolic fermentation. A candidate gene approach was used attempting to identify genes which may be responsible for this adaptation. ESTs sequences from the Genomic Rosaceae Database (GRD) and ESTree database were downloaded. After performing analysis with different bioinformatic programs, tentative consensus sequences were obtained by specific oligo design. Results of cloning and characterization of a low oxygen gene PDC differentially expressed and validated by qRT-PCR under hypoxia conditions in the sensitive almond x peach hybrid 'Felinem' and the tolerant genotype myrobalan plum 'P.2175' are presented.
引用
收藏
页码:135 / 138
页数:4
相关论文
共 11 条
[1]   Molecular biology, biotechnology and genomics of flooding-associated low O2 stress response in plants [J].
Agarwal, S ;
Grover, A .
CRITICAL REVIEWS IN PLANT SCIENCES, 2006, 25 (01) :1-21
[2]  
Amador ML, 2009, ACTA HORTIC, V814, P715
[3]   INVESTIGATION OF THE COFACTOR-BINDING SITE OF ZYMOMONAS-MOBILIS PYRUVATE DECARBOXYLASE BY SITE-DIRECTED MUTAGENESIS [J].
CANDY, JM ;
DUGGLEBY, RG .
BIOCHEMICAL JOURNAL, 1994, 300 :7-13
[4]   Molecular strategies for improving waterlogging tolerance in plants [J].
Dennis, ES ;
Dolferus, R ;
Ellis, M ;
Rahman, M ;
Wu, Y ;
Hoeren, FU ;
Grover, A ;
Ismond, KP ;
Good, AG ;
Peacock, WJ .
JOURNAL OF EXPERIMENTAL BOTANY, 2000, 51 (342) :89-97
[5]   CATALYTIC CENTERS IN THE THIAMIN DIPHOSPHATE DEPENDENT ENZYME PYRUVATE DECARBOXYLASE AT 2.4-ANGSTROM RESOLUTION [J].
DYDA, F ;
FUREY, W ;
SWAMINATHAN, S ;
SAX, M ;
FARRENKOPF, B ;
JORDAN, F .
BIOCHEMISTRY, 1993, 32 (24) :6165-6170
[6]   Characterization of pyruvate decarboxylase genes from rice [J].
Hossain, MA ;
Huq, E ;
Grover, A ;
Dennis, ES ;
Peacock, WJ ;
Hodges, TK .
PLANT MOLECULAR BIOLOGY, 1996, 31 (04) :761-770
[7]   The Pyruvate decarboxylase1 gene of Arabidopsis is required during anoxia but not other environmental stresses [J].
Kürsteiner, O ;
Dupuis, I ;
Kuhlemeier, C .
PLANT PHYSIOLOGY, 2003, 132 (02) :968-978
[8]   Global transcription profiling reveals comprehensive insights into hypoxic response in Arabidopsis [J].
Liu, FL ;
VanToai, T ;
Moy, LP ;
Bock, G ;
Linford, LD ;
Quackenbush, J .
PLANT PHYSIOLOGY, 2005, 137 (03) :1115-1129
[9]   Comparative study between two strawberry pyruvate decarboxylase genes along fruit development and ripening, post-harvest and stress conditions [J].
Moyano, E ;
Encinas-Villarejo, S ;
López-Ráez, JA ;
Redondo-Nevado, J ;
Blanco-Portales, R ;
Bellido, ML ;
Sanz, C ;
Caballero, JL ;
Muñoz-Blanco, J .
PLANT SCIENCE, 2004, 166 (04) :835-845
[10]   PURIFICATION AND CHARACTERIZATION OF PYRUVATE DECARBOXYLASE FROM PEA-SEEDS (PISUM-SATIVUM CV MIKO) [J].
MUCKE, U ;
KONIG, S ;
HUBNER, G .
BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1995, 376 (02) :111-117