Rapid method for the quantification of hydroquinone concentration: chemiluminescent analysis

被引：8

作者：

Chen, Tung-Sheng ^{[1
,2
]}

Liou, Show-Yih ^{[3
]}

Kuo, Wei-Wen ^{[4
]}

Wu, Hsi-Chin ^{[5
]}

Jong, Gwo-Ping ^{[6
]}

Wang, Hsueh-Fang ^{[7
]}

Shen, Chia-Yao ^{[8
]}

Padma, V. Vijaya ^{[9
]}

Huang, Chih-Yang ^{[1
,10
,11
]}

Chang, Yen-Lin ^{[12
]}

机构：

[1] China Med Univ, Grad Inst Basic Med Sci, Taichung 404, Taiwan

[2] China Med Univ, Biomat Translat Res Ctr, Taichung 404, Taiwan

[3] Formosan Blood Purificat Fdn, Taipei, Taiwan

[4] China Med Univ, Dept Biol Sci & Technol, Taichung 404, Taiwan

[5] China Med Univ, Sch Med, Taichung 404, Taiwan

[6] Armed Force Taichung Gen Hosp, Div Cardiol, Taichung, Taiwan

[7] Hungkuang Univ, Inst Biomed Nutr, Taichung 433, Taiwan

[8] Meiho Univ, Dept Nursing, Pingtung 921, Taiwan

[9] Bharathiar Univ, Dept Biotechnol, Coimbatore 641046, Tamil Nadu, India

[10] Asia Univ, Dept Hlth & Nutr Biotechnol, Taichung 413, Taiwan

[11] China Med Univ, Sch Chinese Med, Taichung 404, Taiwan

[12] Chung Yuan Christian Univ, Dept Biomed Engn, Chungli 32023, Taiwan

来源：

LUMINESCENCE | 2015年 / 30卷 / 07期

关键词：

hydroquinone; chemiluminescence; topical; skin whitener; PERFORMANCE LIQUID-CHROMATOGRAPHY;

D O I：

10.1002/bio.2842

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Topical hydroquinone serves as a skin whitener and is usually available in cosmetics or on prescription based on the hydroquinone concentration. Quantification of hydroquinone content therefore becomes an important issue in topical agents. High-performance liquid chromatography (HPLC) is the commonest method for determining hydroquinone content in topical agents, but this method is time-consuming and uses many solvents that can become an environmental issue. We report a rapid method for quantifying hydroquinone content by chemiluminescent analysis. Hydroquinone induces the production of hydrogen peroxide in the presence of basic compounds. Hydrogen peroxide induced by hydroquinone oxidized light-emitting materials such as lucigenin, resulted in the production of ultra-weak chemiluminescence that was detected by a chemiluminescence analyzer. The intensity of the chemiluminescence was found to be proportional to the hydroquinone concentration. We suggest that the rapid (measurement time, 60 s) and virtually solvent-free (solvent volume, <2mL) chemiluminescent method described here for quantifying hydroquinone content may be an alternative to HPLC analysis. Copyright (C) 2015 John Wiley & Sons, Ltd.

引用

页码：947 / 949

页数：3

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