Selective binding of mannose-encapsulated gold nanoparticles to type 1 pili in Escherichia coli

被引：254

作者：

Lin, CC ^{[1
]}

Yeh, YC

Yang, CY

Chen, CL

Chen, GF

Chen, CC

Wu, YC

机构：

[1] Acad Sinica, Inst Chem, Taipei 115, Taiwan

[2] Natl Taiwan Normal Univ, Dept Chem, Taipei 11623, Taiwan

[3] Natl Taiwan Univ, Dept Zool, Taipei 106, Taiwan

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2002年 / 124卷 / 14期

关键词：

D O I：

10.1021/ja0200903

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The synthesis, characterization and biological application of mannose encapsulated gold nanoparticles (m-AuNP) are reported. m-AuNP is well dispersed and very stable without aggregation in the media of broad ion strength and pH ranges. The selective binding of m-AuNP to the mannose adhesin FimH of bacterial type 1 pili is demonstrated using transmission electron microscopy. The competition assay with free mannose suggests that m-AuNP binds FimH better than free mannose does. This work demonstrates that carbohydrate attached nanoparticles can be used as an efficient affinity label and a multi-ligand carrier in a biological system. Copyright © 2002 American Chemical Society.

引用

页码：3508 / 3509

页数：2

共 32 条

[1] Organization of 'nanocrystal molecules' using DNA [J].