Parameters for plaque formation in the potency assay of Japanese measles vaccines

Parameters for plaque formation by measles vaccine strains licensed in Japan were studied. For the plaque test, inoculum volume was one of the critical factors for obtaining an appropriate titre of the sample, A linear relationship between the inoculum volume and the apparent reciprocal titre was discovered, enabling the comparison of absolute titres. Another factor affecting the infectivity was the strain-specific temperature sensitivity in the plaque assay. Although all the vaccine strains tested showed the highest titre at 35 degrees C, the pattern of the temperature sensitivity differed from one strain to another. These factors must be taken into consideration in order to obtain an appropriate titre of a vaccine virus.