Protective effect of whey protein hydrolysates on H2O2-induced PC12 cells oxidative stress via a mitochondria-mediated pathway

被引：74

作者：

Jin, Man-Man ^{[1
]}

Zhang, Li ^{[2
]}

Yu, Hui-Xin ^{[2
]}

Meng, Jun ^{[1
]}

Sun, Zhen ^{[1
]}

Lu, Rong-Rong ^{[1
]}

机构：

[1] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

[2] Minist Hlth, Jiangsu Inst Nucl Med, Key Lab Nucl Med, Wuxi 214063, Jiangsu, Peoples R China

来源：

FOOD CHEMISTRY | 2013年 / 141卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Antioxidant peptides; H2O2; PC12; Apoptosis; Mitochondria-mediated pathway; PEROXIDE-INDUCED APOPTOSIS; CYTOCHROME-C RELEASE; AMYLOID BETA-PEPTIDE; BCL-X-L; IN-VITRO; ACTIVATION; DEATH; NECROSIS; CASEIN; CED-3;

D O I：

10.1016/j.foodchem.2013.03.076

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Whey protein hydrolysates (WPHs) were prepared with pepsin and trypsin. A PC12 cell model was built to observe the protective effect of WPHs against H2O2-induced oxidative stress. The results indicated that WPHs reduced apoptosis by 14% and increased antioxidant enzyme activities. Flow cytometry was used to assess the accumulation of reactive oxygen species (ROS), Ca2+ levels and the mitochondria( membrane potential (MMP). The results showed that WPHs suppressed ROS elevation and Ca2+ levels and stabilised MMP by 16%. The anti-apoptosis/pro-apoptosis proteins Bcl-2/Bax and poly (ADP-ribose) polymerase (PARP) were investigated by Western-blot analysis, which indicated that WPHs increased the expression of Bcl-2 while inhibiting the expression of Bax and the degradation of PARP. WPHs also blocked Caspase-3 activation by 62%. The results demonstrate that WPHs can significantly protect PC12 cells against oxidative stress via a mitochondria-mediated pathway. These findings indicate the potential benefits of WPHs as valuable food antioxidative additives. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：847 / 852

页数：6

共 27 条

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