Fluorescence Lifetime Imaging Microscopy for the Monitoring of Green Fluorescent Protein-Tagged Androgen Receptors in Living Cells

被引：2

作者：

Miyake, Rina ^{[1
]}

Uchimura, Tomohiro ^{[1
,2
,3
]}

Li, Xu ^{[1
,4
]}

Imasaka, Totaro ^{[1
,2
]}

机构：

[1] Kyushu Univ, Grad Sch Engn, Dept Appl Chem, Fukuoka 8190395, Japan

[2] Kyushu Univ, Ctr Future Chem, Dept Translat Res Ctr, Nishi Ku, Fukuoka 8190395, Japan

[3] Univ Fukui, Grad Sch Engn, Dept Mat Sci & Engn, Bunkyo Ku, Fukui 9108507, Japan

[4] China Med Univ, Affiliated Hosp 1, Dept Orthoped Surg, Shenyang 110001, Peoples R China

来源：

CHEMICAL & PHARMACEUTICAL BULLETIN | 2013年 / 61卷 / 01期

关键词：

androgen receptor; 5; alpha-dihydrotestosterone; hydroxyflutamide; fluorescence lifetime imaging microscopy; green fluorescent protein; STEROID-HORMONE RECEPTORS; BINDING; KINETICS;

D O I：

10.1248/cpb.c12-00428

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Fluorescence lifetime imaging microscopy (FLIM) was used to monitor the interaction between androgen receptor (AR) tagging of a green fluorescent protein (GFP) and the ligands in living cells. The fluorescence lifetime of the AR-GFP without ligands was ca. 3.1ns, which was reduced to ca. 2.5ns after treatment with agonist 5 alpha-dihydrotestosterone. On the other hand, the fluorescence lifetime of AR-GFP was not changed after treatment with antagonist hydroxyflutamide. The reaction kinetics was simulated in the present study, and the obtained results indicated the possibility of the presence of an intermediate complex during the reaction. FLIM can be used to record the ratio of the AR as it reacts with an agonist, and, therefore, it is useful for acquiring information concerning the interaction between AR and ligands in living cells.

引用

页码：82 / 84

页数：3

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