Synthesis of branched polysaccharides with tunable degree of branching

被引：50

作者：

Ciric, Jelena ^{[1
]}

Loos, Katja ^{[1
]}

机构：

[1] Univ Groningen, Zernike Inst Adv Mat, Dept Polymer Chem, NL-9747 AG Groningen, Netherlands

来源：

CARBOHYDRATE POLYMERS | 2013年 / 93卷 / 01期

关键词：

Degree of branching; Glycogen branching enzyme; Phosphorylase; Polysaccharides; GLYCOGEN-PHOSPHORYLASE; GLUCAN PHOSPHORYLASE; SUBSTRATE-BINDING; PURIFICATION; ENZYMES; POTATO; MUSCLE;

D O I：

10.1016/j.carbpol.2012.04.008

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

An in vitro enzyme-catalyzed tandem reaction using the enzymes phosphorylase b from rabbit muscle and Deinococcus geothermalis glycogen branching enzyme (Dg GBE) to obtain branched polyglucans with tunable degree of branching (2% divided by 13%) is presented. The tunable degree of branching is obtained by varying the reaction conditions such as pH value, the choice of reducing agent and its concentration and reaction time. Linear amylose is formed by the phosphorylase-catalyzed propagation of glucose1-phosphate while Dg GBE introduces branching points on the alpha(1 -> 6) position by relocating short oligosaccharide chains. Our results show that the best way to obtain different degrees of branching with this set of enzymes is by regulation of the reaction time. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：31 / 37

页数：7

共 34 条

[11] POTATO AND RABBIT MUSCLE PHOSPHORYLASES - COMPARATIVE STUDIES ON THE STRUCTURE, FUNCTION AND REGULATION OF REGULATORY AND NON-REGULATORY ENZYMES [J].

FUKUI, T ;

SHIMOMURA, S ;

NAKANO, K .

MOLECULAR AND CELLULAR BIOCHEMISTRY, 1982, 42 (03) :129-144

[12] A comparison between the sulfhydryl reductants tris(2-carboxyethyl)phosphine and dithiothreitol for use in protein biochemistry [J].

Getz, EB ;

Xiao, M ;

Chakrabarty, T ;

Cooke, R ;

Selvin, PR .

ANALYTICAL BIOCHEMISTRY, 1999, 273 (01) :73-80

[13]

GOLDEMBERG SH, 1962, BIOCHIM BIOPHYS ACTA, V56, P357, DOI 10.1016/0006-3002(62)90576-0

[14] Thermal denaturation pathway of starch phosphorylase from Corynebacterium callunae:: Oxyanion binding provides the glue that efficiently stabilizes the dimer structure of the protein [J].

Griessler, R ;

D'Auria, S ;

Tanfani, F ;

Nidetzky, B .

PROTEIN SCIENCE, 2000, 9 (06) :1149-1161

[15] A PROCEDURE FOR QUANTITATIVE-DETERMINATION OF TRIS(2-CARBOXYETHYL)PHOSPHINE, AN ODORLESS REDUCING AGENT MORE STABLE AND EFFECTIVE THAN DITHIOTHREITOL [J].

HAN, JC ;

HAN, GY .

ANALYTICAL BIOCHEMISTRY, 1994, 220 (01) :5-10

[16] CALORIMETRIC STUDY OF INTERACTIONS BETWEEN PHOSPHORYLASE-B AND ITS NUCLEOTIDE ACTIVATORS [J].

HO, HC ;

WANG, JH .

BIOCHEMISTRY, 1973, 12 (23) :4750-4755

[17] Amylomaltase of Pyrobaculum aerophilum IM2 produces thermoreversible starch gels [J].

Kaper, T ;

Talik, B ;

Ettema, TJ ;

Bos, H ;

van der Maarel, MJEC ;

Dijkhuizen, L .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (09) :5098-5106

[18] Enzymatic polymerization [J].

Kobayashi, S ;

Uyama, H ;

Kimura, S .

CHEMICAL REVIEWS, 2001, 101 (12) :3793-3818

[19] Glucan synthesis in the genus Lactobacillus:: isolation and characterization of glucansucrase genes, enzymes and glucan products from six different strains [J].

Kralj, S ;

van Geel-Schutten, GH ;

Dondorff, MMG ;

Kirsanovs, S ;

van der Maarel, MJEC ;

Dijkhuizen, L .

MICROBIOLOGY-SGM, 2004, 150 :3681-3690

[20]

Ktatz R. F., 1980, METHODS EXPT PHYS

← 1 2 3 4 →