Synthesis of branched polysaccharides with tunable degree of branching

被引：50

作者：

Ciric, Jelena ^{[1
]}

Loos, Katja ^{[1
]}

机构：

[1] Univ Groningen, Zernike Inst Adv Mat, Dept Polymer Chem, NL-9747 AG Groningen, Netherlands

来源：

CARBOHYDRATE POLYMERS | 2013年 / 93卷 / 01期

关键词：

Degree of branching; Glycogen branching enzyme; Phosphorylase; Polysaccharides; GLYCOGEN-PHOSPHORYLASE; GLUCAN PHOSPHORYLASE; SUBSTRATE-BINDING; PURIFICATION; ENZYMES; POTATO; MUSCLE;

D O I：

10.1016/j.carbpol.2012.04.008

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

An in vitro enzyme-catalyzed tandem reaction using the enzymes phosphorylase b from rabbit muscle and Deinococcus geothermalis glycogen branching enzyme (Dg GBE) to obtain branched polyglucans with tunable degree of branching (2% divided by 13%) is presented. The tunable degree of branching is obtained by varying the reaction conditions such as pH value, the choice of reducing agent and its concentration and reaction time. Linear amylose is formed by the phosphorylase-catalyzed propagation of glucose1-phosphate while Dg GBE introduces branching points on the alpha(1 -> 6) position by relocating short oligosaccharide chains. Our results show that the best way to obtain different degrees of branching with this set of enzymes is by regulation of the reaction time. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：31 / 37

页数：7

共 34 条

[1] STRUCTURAL MECHANISM FOR GLYCOGEN-PHOSPHORYLASE CONTROL BY PHOSPHORYLATION AND AMP [J].

BARFORD, D ;

HU, SH ;

JOHNSON, LN .

JOURNAL OF MOLECULAR BIOLOGY, 1991, 218 (01) :233-260

[2] BIOSYNTHESIS OF BACTERIAL GLYCOGEN - PURIFICATION AND PROPERTIES OF ESCHERICHIA-COLI-B ALPHA-1,4-GLUCAN-ALPHA-1,4-GLUCAN 6-GLYCOSYLTRANSFERASE [J].

BOYER, C ;

PREISS, J .

BIOCHEMISTRY, 1977, 16 (16) :3693-3699

[3]

BRANDT H, 1975, J BIOL CHEM, V250, P8038

[4] EXAMINATION OF THE SECONDARY STRUCTURE OF PROTEINS BY DECONVOLVED FTIR SPECTRA [J].

BYLER, DM ;

SUSI, H .

BIOPOLYMERS, 1986, 25 (03) :469-487

[5] DITHIOTHREITOL NEW PROTECTIVE REAGENT FOR SH GROUPS [J].

CLELAND, WW .

BIOCHEMISTRY, 1964, 3 (04) :480-&

[6]

CORI GERTY T., 1939, JOUR BIOL CHEM, V131, P397

[7]

Cori GT, 1936, J BIOL CHEM, V116, P129

[8]

Cori GT, 1940, J BIOL CHEM, V135, P733

[9] P-31 NUCLEAR MAGNETIC-RESONANCE STUDIES OF GLYCOGEN-PHOSPHORYLASE FROM RABBIT SKELETAL-MUSCLE - IONIZATION STATES OF PYRIDOXAL 5'-PHOSPHATE [J].

FELDMANN, K ;

HULL, WE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (03) :856-860

[10] Bioengineering and application of novel glucose polymers [J].

Fujii, K ;

Takata, H ;

Yanase, M ;

Terada, Y ;

Ohdan, K ;

Takaha, T ;

Okada, S ;

Kuriki, T .

BIOCATALYSIS AND BIOTRANSFORMATION, 2003, 21 (4-5) :167-172

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