Role of ROS modified human DNA in the pathogenesis and etiology of cancer

被引：72

作者：

Abdi, S ^{[1
]}

Ali, A ^{[1
]}

机构：

[1] Aligarh Muslim Univ, JN Med Coll, Dept Biochem, Aligarh 202002, Uttar Pradesh, India

来源：

CANCER LETTERS | 1999年 / 142卷 / 01期

关键词：

reactive oxygen species; human DNA; cancer;

D O I：

10.1016/S0304-3835(99)00112-3

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The effect of hydroxyl radical, generated by ultraviolet (UV) irradiation of hydrogen peroxide, on human placental DNA was monitored by UV spectroscopy, melting temperature studies, S-1 nuclease digestibility and hydroxyapatite column chromatography. Immunological data indicated that reactive oxygen species (ROS) modified human DNA induced high titer antibodies. In ELISA, serum antibodies from various cancer patients showed a higher recognition of ROS-human DNA as compared to native DNA. Retarded mobility of the immune complex formed between IgG, isolated from cancer sera, and ROS-human DNA provided convincing evidence for antigen-antibody interaction. Oxidative lesions in DNA of cancer patients were probed using anti-ROS-human DNA IgG. DNA from cancer patients were found to inhibit anti-ROS-human DNA IgG activity in the range of 40% to 57%. These binding results indicate the presence of oxidative lesions in the cancer patient's genome. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：1 / 9

页数：9

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