Elucidation of degrading pattern and substrate recognition of a novel bifunctional alginate lyase from Flammeovirga sp. NJ-04 and its use for preparation alginate oligosaccharides

被引：68

作者：

Zhu, Benwei ^{[1
]}

Ni, Fang ^{[1
]}

Sun, Yun ^{[1
]}

Ning, Limin ^{[2
]}

Yao, Zhong ^{[1
]}

机构：

[1] Nanjing Tech Univ, Coll Food Sci & Light Ind, 30 Puzhu Rd, Nanjing 211816, Jiangsu, Peoples R China

[2] Nanjing Univ Chinese Med, Coll Med & Life Sci, Nanjing 210023, Jiangsu, Peoples R China

来源：

BIOTECHNOLOGY FOR BIOFUELS | 2019年 / 12卷

基金：

中国国家自然科学基金;

关键词：

Alginate lyase; Bifunctional; Oligosaccharide; Action pattern; Substrate recognition; MAJOR SOURCES; VIBRIO; PURIFICATION; BACTERIUM; DEGRADATION; CLONING; STRAIN; POLY(ALPHA-L-GULURONATE)LYASE; CLASSIFICATION; AGARIVORANS;

D O I：

10.1186/s13068-019-1352-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

BackgroundThe alginate oligosaccharides have been widely used in agriculture, medicine, and food industries due to their versatile physiological functions such as antioxidant, anticoagulant, and antineoplastic activities. The bifunctional alginate lyases can degrade the alginate polysaccharide more efficiently into alginate oligosaccharides. Therefore, it is crucial to discover new bifunctional alginate lyase for alginate oligosaccharide production.ResultsHerein, a novel bifunctional alginate lyase FsAlgB was cloned and identified from deep-sea bacterium Flammeovirga sp. NJ-04, which exhibited broad substrate specificity and the highest activity (1760.8U/mg) at pH 8.0 and 40 degrees C. Furthermore, the K-m values of FsAlgB towards polyG (0.69mM) and polyMG (0.92mM) were lower than that towards sodium alginate (1.28mM) and polyM (2.06mM). Recombinant FsAlgB was further characterized as an endolytic alginate lyase, and it can recognize the tetrasaccharide as the minimal substrate and cleave the glycosidic bonds between the subsites of -3 and +1.ConclusionThis study provided extended insights into the substrate recognition and degrading pattern of alginate lyases with broad substrate specificity.

引用

页数：13

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