Large Changes in Protonation of Weak Polyelectrolyte Brushes with Salt Concentration-Implications for Protein Immobilization

被引:35
作者
del Castillo, Gustav Ferrand-Drake [1 ]
Hailes, Rebekah L. N. [1 ]
Dahlin, Andreas [1 ]
机构
[1] Chalmers Univ Technol, Dept Chem & Chem Engn, S-41296 Gothenburg, Sweden
基金
瑞典研究理事会;
关键词
POLY(ACRYLIC ACID); POLYMER BRUSH; HIGH-CAPACITY; ADSORPTION; BEHAVIOR; ENZYMES; BINDING;
D O I
10.1021/acs.jpclett.0c01289
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
We report for the first time that the protonation behavior of weak polyelectrolyte brushes depends very strongly on ionic strength. The pK, changes by one pH step per order of magnitude in salt concentration. For low salt concentrations (similar to 1 mM), a very high pH is required to deprotonate a polyacidic brush and a very low pH is required to protonate a polybasic brush. This has major consequences for interactions with other macromolecules, as the brushes are actually almost fully neutral when believed to be charged. We propose that many previous studies on electrostatic interactions between polyelectrolytes and proteins have, in fact, looked at other types of intermolecular forces, in particular, hydrophobic interactions and hydrogen bonds.
引用
收藏
页码:5212 / 5218
页数:7
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