Multilineage gene expression precedes commitment in the hemopoietic system

被引：596
作者：
Hu, M
Krause, D
Greaves, M
Sharkis, S
Dexter, M
Heyworth, C
Enver, T
机构：
[1] INST CANC RES, CHESTER BEATTY LABS, LEUKAEMIA RES FUND CTR, LONDON SW3 6JB, ENGLAND
[2] YALE UNIV, DEPT LAB MED, NEW HAVEN, CT 06520 USA
[3] JOHNS HOPKINS UNIV, CTR ONCOL, BALTIMORE, MD 21287 USA
[4] CHRISTIE HOSP & HOLT RADIUM INST, PATERSON INST CANC RES, MANCHESTER M20 9BX, LANCS, ENGLAND
来源：
GENES & DEVELOPMENT | 1997年 / 11卷 / 06期
关键词：
hemopoietic stem cells; lineage specification; cytokine receptors;
D O I：
10.1101/gad.11.6.774
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
We have tested the hypothesis that multipotential hemopoietic stem and progenitor cells prime several different lineage-affiliated programs of gene activity prior to unilineage commitment and differentiation. Using single cell RT-PCR we show that erythroid (beta-globin) and myeloid (myeloperoxidase) gene expression programs can be initiated by the same cell prior to exclusive commitment to the erythroid or granulocytic lineages. furthermore, the multipotential state is characterized by the coexpression of several lineage-affiliated cytokine receptors. These data support a model of hemopoietic lineage specification in which unilineage commitment is prefaced by a ''promiscuous'' phase of multilineage locus activation.
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收藏
页码：774 / 785
页数：12
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