NMR of peptides and proteins in oriented membranes

Solid-state NMR spectroscopy is used to determine the structures of membrane peptides and proteins in lipid bilayers. The methodology for membrane protein structure determination using solid-state NMR of oriented lipid bilayer samples is outlined. Recent developments in recombinant bacterial expression systems for the preparation of isotopically labeled membrane proteins, pulse sequences for high-resolution spectroscopy, and structural indices that guide the structure assembly process greatly extend the capabilities of the technique. (C) 2002 Wiley Periodicals, Inc.