FTIR, 1H NMR and EPR spectroscopy studies on the interaction of flavone apigenin with dipalmitoylphosphatidylcholine liposomes

被引:93
作者
Pawlikowska-Pawlega, Bozena [1 ]
Misiak, Lucjan E. [2 ]
Zarzyka, Barbara [3 ]
Paduch, Roman [4 ]
Gawron, Antoni [1 ]
Gruszecki, Wieslaw I. [5 ]
机构
[1] Marie Curie Sklodowska Univ, Dept Comparat Anat & Anthropol, PL-20033 Lublin, Poland
[2] Marie Curie Sklodowska Univ, Inst Phys, Dept Surface Phys & Nanostruct, PL-20031 Lublin, Poland
[3] Marie Curie Sklodowska Univ, Dept Cell Biol, PL-20033 Lublin, Poland
[4] Marie Curie Sklodowska Univ, Dept Virol & Immunol, PL-20033 Lublin, Poland
[5] Marie Curie Sklodowska Univ, Inst Phys, Dept Biophys, PL-20031 Lublin, Poland
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2013年 / 1828卷 / 02期
关键词
Apigenin; DPPC liposome; H-1; NMR; EPR; FTIR; Electron microscopy; CELL-CYCLE ARREST; ANTIBIOTIC AMPHOTERICIN-B; ELECTRON-SPIN-RESONANCE; LIPID-MEMBRANES; ENDOPLASMIC-RETICULUM; QUERCETIN; LOCALIZATION; MODULATION; ACTIVATION; INDUCTION;
D O I
10.1016/j.bbamem.2012.10.013
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Apigenin (5,7,4'-trihydroxyflavone) is a cancer chemopreventive agent and a member of the family of plant flavonoids. Apigenin interaction with liposomes formed with dipalmitoylphosphatidylcholine (DPPC) was investigated by means of FTIR spectroscopy, H-1 NMR and EPR techniques. Fluorescent microscopy and electron microscopy were applied to study the apigenin effects on colon myofibroblasts and human skin fibroblasts. The strong rigidifying effect of apigenin with respect to polar head groups was concluded on the basis of the action of the flavone on partition coefficient of Tempo spin label between the water and lipid phases. The ordering effect was also found in hydrophobic region at the depth monitored by 5-SASL and 16-SASL spin labels. The inclusion of apigenin to the membrane restricted the motional freedom of polar head groups lowering penetration of Pr3+ ions to the membranes. The H-1 NMR technique supported also the restriction of motional freedom of the membrane in the hydrophobic region, especially in the zone of CH2 groups of alkyl chains. FTIR analysis showed that apigenin incorporates into DPPC liposomes via hydrogen bonding between its own hydroxyl groups and lipid polar head groups in the C-O-P-O-C segment. It is also very likely that hydroxyl groups of apigenin link with polar groups of DPPC by water bridges. Electron and fluorescence microscopic observations revealed changes in the internal membrane organization of the examined cells. In conclusion, the changes of the structural and dynamic properties of membranes can be crucial for processes involving tumor suppression signal transduction pathways and cell cycle regulation. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:518 / 527
页数:10
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