Inhibition Mechanisms of Hepatitis C Virus Infection by Caffeic Acid and Tannic Acid

被引：18

作者：

Shirasago, Yoshitaka ^{[1
]}

Inamori, Yoko ^{[1
]}

Suzuki, Takeru ^{[1
,2
]}

Tanida, Isei ^{[3
]}

Suzuki, Tetsuro ^{[4
]}

Sugiyama, Kazuo ^{[5
]}

Wakita, Takaji ^{[6
]}

Hanada, Kentaro ^{[1
]}

Fukasawa, Masayoshi ^{[1
]}

机构：

[1] Natl Inst Infect Dis, Dept Biochem & Cell Biol, Shinjuku Ku, 1-23-1 Toyama, Tokyo 1628640, Japan

[2] Tokyo Univ Sci, Fac Sci, Dept Chem, Shinjuku Ku, 1-3 Kagurazaka, Tokyo 1628601, Japan

[3] Juntendo Univ, Grad Sch Med, Dept Cell Biol & Neurosci, Bunkyo Ku, 2-1-1 Hongo, Tokyo 1138421, Japan

[4] Hamamatsu Univ Sch Med, Dept Infect Dis, Higashi Ku, 1-20-1 Handayama, Hamamatsu, Shizuoka 4313192, Japan

[5] Gyotoku Gen Hosp, 5525-2 Hongyotoku, Ichikawa, Chiba 2720103, Japan

[6] Natl Inst Infect Dis, Dept Virol 2, Shinjuku Ku, 1-23-1 Toyama, Tokyo 1628640, Japan

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2019年 / 42卷 / 05期

关键词：

hepatitis C virus; caffeic acid; tannic acid; apolipoprotein E; HUMAN APOLIPOPROTEIN-E; HCV; MULTIPLICATION; INTERFERON; CAPACITY;

D O I：

10.1248/bpb.b18-00970

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Previously, we reported that coffee extract and its constituents, caffeic acid (CA) and p-coumaric acid, inhibit infection by the hepatitis C virus (HCV). In the present report, we identified another coffee-related compound, tannic acid (TA), which also inhibits HCV infection. We systematically evaluated which steps of the viral lifecycle were affected by CA and TA. TA substantially inhibits HCV RNA replication and egression, while CA does not. The infectivity of the HCV pretreated with CA or TA was almost lost. Cellular attachment of HCV particles and their interaction with apolipoprotein E, which is essential for HCV infectivity, were significantly reduced by CA. These results indicate that CA inhibits HCV entry via its direct effect on viral particles and TA inhibits HCV RNA replication and particle egression as well as entry into host cells. Taken together, our findings may provide insights into CA and TA as potential anti-HCV strategies.

引用

页码：770 / 777

页数：8

共 39 条

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