Electrochemical monitoring of DNA hybridization by multiwalled carbon nanotube based screen printed electrodes

被引：49

作者：

Karadeniz, Hakan ^{[1
]}

Erdem, Arzum ^{[1
]}

Caliskan, Ayfer ^{[1
]}

机构：

[1] Ege Univ, Fac Pharm, Dept Analyt Chem, TR-35100 Izmir, Turkey

来源：

ELECTROANALYSIS | 2008年 / 20卷 / 17期

关键词：

DNA; DNA hybridization; multiwalled carbon nanotubes; screen printed electrodes; guanine signal; Hepatitis B virus (HBV);

D O I：

10.1002/elan.200804270

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The application of multiwalled carbon nanotube (MWCNT) based screen printed graphite electrodes (SPEs) was explored in this study for the electrochemical monitoring of DNA hybridization related to specific sequences on Hepatitis B virus (HBV) DNA. After the microscopic characterization of bare MWCNT-SPEs and DNA immobilized ones was performed, the optimization of assay has been studied. The development of screen printing process combined with nanomaterial based disposable sensor technology leads herein a great opportunity for DNA detection using differential pulse voltammetry (DPV) by measuring the guanine oxidation signal observed at + 1.00 V in the presence of DNA hybridization between HBV probe and its complementary, target. The detection limit estimated for signal to noise ratios = 3 corresponds to 96.33 nM target concentration in the 40 mu L samples. The advantages of carbon nanotube based screen printed electrode used for electrochemical monitoring of DNA hybridization are discussed with sensitivity, selectivity and reproducibility in comparison with previous nanomaterial based electrochemical transducers developed for DNA or other biomolecular recognitions.

引用

页码：1932 / 1938

页数：7

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