Highly selective detection of 5-methylcytosine using photochemical ligation

被引：14

作者：

Ogino, Masayuki ^{[1
]}

Taya, Yuuta ^{[2
]}

Fujimoto, Kenzo ^{[1
,2
]}

机构：

[1] Japan Sci & Technol Agcy, Ishikawa 9231211, Japan

[2] Japan Adv Inst Sci & Technol, Sch Mat Sci, Ishikawa 9231292, Japan

来源：

CHEMICAL COMMUNICATIONS | 2008年 / 45期

关键词：

D O I：

10.1039/b813677g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report the nonenzymatic detection of 5-methylcytosine by using template-directed photoligation through 5-cyanovinyl-2'- deoxyuridine (U-C) with high selectivity and present a new methylation detection method using a photoligation-based DNA chip assay.

引用

收藏

页码：5996 / 5998

页数：3

相关论文

共 25 条

[1] Selective detection of 5-methylcytosine sites in DNA [J].

Bareyt, Sebastian ;

Carell, Thomas .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2008, 47 (01) :181-184

[2] Analysis of DNA-microarrays produced by inverse in situ oligonucleotide synthesis [J].

Beier, M ;

Hoheisel, JD .

JOURNAL OF BIOTECHNOLOGY, 2002, 94 (01) :15-22

[3] Methylation-dependent fragment separation: Direct detection of DNA methylation by capillary electrophoresis of PCR products from bisulfite-converted genomic DNA [J].

Boyd, Victoria L. ;

Moody, Kristina I. ;

Karger, Achim E. ;

Livak, Kenneth J. ;

Zon, Gerald ;

Burns, John W. .

ANALYTICAL BIOCHEMISTRY, 2006, 354 (02) :266-273

[4] Quantification of oxidative single-base and intrastrand cross-link lesions in unmethylated and CpG-methylated DNA induced by Fenton-type reagents [J].

Cao, Huachuan ;

Wang, Yinsheng .

NUCLEIC ACIDS RESEARCH, 2007, 35 (14) :4833-4844

[5] A GENOMIC SEQUENCING PROTOCOL THAT YIELDS A POSITIVE DISPLAY OF 5-METHYLCYTOSINE RESIDUES IN INDIVIDUAL DNA STRANDS [J].

FROMMER, M ;

MCDONALD, LE ;

MILLAR, DS ;

COLLIS, CM ;

WATT, F ;

GRIGG, GW ;

MOLLOY, PL ;

PAUL, CL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (05) :1827-1831

[6] Photoinduced DNA end capping via N3-methyl-5-cyanovinyl-2′-deoxyuridine [J].

Fujimoto, K ;

Yoshimura, Y ;

Ikemoto, T ;

Nakazawa, A ;

Hayashi, M ;

Saito, I .

CHEMICAL COMMUNICATIONS, 2005, (25) :3177-3179

[7] Template-directed photoreversible ligation of deoxyoligonucleotides via 5-vinyldeoxyuridine [J].

Fujimoto, K ;

Matsuda, S ;

Takahashi, N ;

Saito, I .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (23) :5646-5647

[8] Site-specific transition of cytosine to uracil via reversible DNA photoligation [J].

Fujimoto, Kenzo ;

Matsuda, Shigeo ;

Yoshimura, Yoshinaga ;

Matsumura, Takashi ;

Hayashi, Masayuki ;

Saito, Isao .

CHEMICAL COMMUNICATIONS, 2006, (30) :3223-3225

[9] Genome-wide profiling of promoter methylation in human [J].

Hatada, I. ;

Fukasawa, M. ;

Kimura, M. ;

Morita, S. ;

Yamada, K. ;

Yoshikawa, T. ;

Yamanaka, S. ;

Endo, C. ;

Sakurada, A. ;

Sato, M. ;

Kondo, T. ;

Horii, A. ;

Ushijima, T. ;

Sasaki, H. .

ONCOGENE, 2006, 25 (21) :3059-3064

[10] The role of DNA methylation in mammalian epigenetics [J].

Jones, PA ;

Takai, D .

SCIENCE, 2001, 293 (5532) :1068-1070