Changes in light sensitivity of photosynthesis in tobacco (Nicotiana tabacum) planes transformed with the antisense gene of glutamate-1-semialdehyde aminotransferase

被引:0
作者
Matorin, DN [1 ]
Pers, I
Hoffmann, P
机构
[1] Moscow MV Lomonosov State Univ, Fac Biol, Dept Biophys, Moscow 119899, Russia
[2] Humboldt Univ, Inst Biol, Dept Plant Biol, Berlin, Germany
关键词
Nicotiana tabacum; transgenic plants; antisense gene of glutamate-1-semialdehyde aminotransferase; chlorophyll synthesis; chlorophyll fluorescence; thermoluminescence; photosystem II; photoinhibition; lipid peroxidation;
D O I
暂无
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Chlorophyll fluorescence and thermoluminescence were used to study photosynthesis in tobacco transgenic plants, in which early stages of chlorophyll synthesis were inhibited owing to the expression of the glutamate-1-semialdehyde aminotransferase gene in the antisense orientation. When such plants were grown in weak light (30 mu Einstein/(m(2) s)), the chlorophyll a content was substantially reduced but the proportion of pigments in light-harvesting complexes was little affected; photosystem II retained its activity, as established from fluorescence parameters. At the same time, changes in the optical properties of leaves in transgenic plants, caused by a reduction in chlorophyll content, enhanced light sensitivity. When these transgenic plants were grown under standard illumination conditions (300 mu Einstein/(m(2)s)), they experienced stress associated with partial photoinhibition of photosystem II activity, enhanced nonphotochemical dissipation of light. energy, elevated rates of lipid peroxidation, and the consequent emission of high-temperature (120 degrees C) thermoluminescence of chlorophyll.
引用
收藏
页码:467 / 473
页数:7
相关论文
共 30 条