TGF-β Signaling Accelerates Senescence of Human Bone-Derived CD271 and SSEA-4 Double-Positive Mesenchymal Stromal Cells

被引:39
作者
Kawamura, Hiroshi [1 ,2 ]
Nakatsuka, Ryusuke [1 ]
Matsuoka, Yoshikazu [1 ]
Sumide, Keisuke [1 ]
Fujioka, Tatsuya [1 ]
Asano, Hiroaki [3 ]
Iida, Hirokazu [2 ]
Sonoda, Yoshiaki [1 ]
机构
[1] Kansai Med Univ, Grad Sch Med Sci, Dept Stem Cell Biol & Regenerat Med, Hirakata, Osaka 5731010, Japan
[2] Kansai Med Univ, Dept Orthopaed Surg, Hirakata, Osaka, Japan
[3] Kyoto Prefectural Univ Med, Sch Nursing, Kyoto, Japan
关键词
HEMATOPOIETIC-STEM; AGING MECHANISMS; NICHE; EXPRESSION; REGULATORS; SUPPORT; QUALITY;
D O I
10.1016/j.stemcr.2018.01.030
中图分类号
Q813 [细胞工程];
学科分类号
摘要
It is generally thought that the proliferative capacity and differentiation potential of somatic stem cells, including mesenchymal stromal/ stem cells (MSCs) and hematopoietic stem cells, decline with age. We investigated the effects of aging on human bone-derived MSCs expressing CD271 and SSEA-4 (double-positive MSCs [DPMSCs]). The percentages of DPMSCs in bone tissue decreased significantly with age. The DPMSCs from elderly patients (old DPMSCs) showed cellular senescence, which was evidenced by low growth potential, high senescence-associated beta-galactosidase activity, and elevated p16 and p21 CDK inhibitor levels. Moreover, old DPMSCs showed weak osteogenic differentiation potential and less hematopoiesis-supporting activity in comparison with young DPMSCs. Interestingly, the addition of transforming growth factor beta 2 (TGF-beta 2) induced cellular senescence in young DPMSCs. With the exception of the adipogenic differentiation potential, all of the aging phenomena observed in old DPMSCs were reversed by the addition of anti-TGF-beta antibodies. These results suggest that, in part, old DPMSCs accelerate cellular senescence through TGF-beta signaling.
引用
收藏
页码:920 / 932
页数:13
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