Increased expression of interleukin-32 in the inflamed ileum of ankylosing spondylitis patients

被引:19
作者
Ciccia, Francesco [1 ]
Rizzo, Aroldo [2 ]
Accardo-Palumbo, Antonina [2 ]
Giardina, AnnaRita [1 ]
Bombardieri, Michele [3 ]
Guggino, Giuliana [1 ]
Taverna, Simona [4 ]
De Leo, Giacomo [4 ]
Alessandro, Riccardo [4 ]
Triolo, Giovanni [1 ]
机构
[1] Univ Palermo, Sez Reumatol, Dipartimento Biomed Med Interna & Specialist, Palermo, Italy
[2] Azienda Osped Osped Riuniti Villa Sofia Cervello, Palermo, Italy
[3] Queen Mary Univ, William Harvey Res Inst, Ctr Expt Med & Rheumatol, London, England
[4] Univ Palermo, Dipartimento Biopatol & Biotecnol Med & Forensi, Palermo, Italy
关键词
IL-32; intestinal inflammation; ankylosing spondylitis; Crohn's disease; Paneth cells; CROHNS-DISEASE; CYTOKINE; NOD2; OVEREXPRESSION; INFLAMMATION; IL-32-GAMMA; INNATE; CELLS; IL-32; GUT;
D O I
10.1093/rheumatology/kes170
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Methods. Quantitative gene expression analysis, by real-time PCR, of IL-32, IL-1 beta, IL-10, TNF-alpha and IFN-gamma was performed on ileal biopsies of 15 AS and 15 Crohn's disease (CD) patients and 10 healthy subjects (HSs). IL-32 tissue distribution was evaluated by immunohistochemistry. The effect of IL-32 on the production of IL-10 by intestinal epithelial cell lines was also evaluated. Results. In the ileal specimens of patients with AS and intestinal chronic inflammation, significant up-regulation of IL-32 at both the mRNA and protein levels was found as compared with non-inflamed AS patients and controls. IL-32 over-expression in AS was accompanied by a significant increase of IL-10 but not of cytokines involved in IL-32 induction. IL-32 stimulates intestinal epithelial cell lines in vitro to produce IL-10. Conclusion. Our findings suggest IL-32 as an important cytokine probably involved in the innate immune response occurring in early phases of intestinal inflammation, where it seems to play a prevalent protective role.
引用
收藏
页码:1966 / 1972
页数:7
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