Cytogenetic analysis of B chromosomes in one population of the fish Moenkhausia sanctaefilomenae (Steindachner, 1907) (Teleostei, Characiformes)

被引：17

作者：

Hashimoto, Diogo Teruo ^{[1
]}

Voltolin, Tatiana Aparecida ^{[1
]}

Valim de Arruda Paes, Ana Danyelle Noitel ^{[1
]}

Foresti, Fausto ^{[2
]}

Bortolozzi, Jehud ^{[1
]}

Porto-Foresti, Fabio ^{[1
]}

机构：

[1] Univ Estadual Paulista UNESP, Fac Ciencias, Dept Ciencias Biol, BR-17033360 Bauru, SP, Brazil

[2] Univ Estadual Paulista UNESP, Inst Biociencias, Dept Morfol, BR-18618000 Botucatu, SP, Brazil

来源：

COMPARATIVE CYTOGENETICS | 2012年 / 6卷 / 02期

基金：

巴西圣保罗研究基金会;

关键词：

fish cytogenetic; NOR expression; supernumerary chromosomes; mitotic instability; NUCLEOLUS ORGANIZER REGIONS; PROCHILODUS-LINEATUS CHARACIFORMES; CHARACIDAE; FREQUENCY; PISCES; TETRAGONOPTERINAE; TRANSCRIPTION;

D O I：

10.3897/CompCytogen.v6i2.1769

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The aim of this study was to characterize cytogenetically one population of the fish Moenkhausia sanctaefilomenae (Steindachner, 1907), with emphasis on the analysis of B chromosomes. The nucleolar activity in the B microchromosomes was characterized, and an analysis of mitotic instability of these microchromosomes was accomplished. The results showed a diploid chromosome number of 50 chromosomes. In all individuals, we observed the presence of B microchromosomes with intra- and inter-individual variability. The analysis of the nucleolus organizing regions (NORs) by silver nitrate staining demonstrated multiple NORs. We observed active sites of ribosomal DNA in the B microchromosomes, with a frequency of 20% in the analyzed cells, which shows gene activity in these chromosomal elements. The analysis of constitutive heterochromatin patterns showed that the B microchromosomes are heterochromatic or euchromatic, which demonstrates differentiation of DNA composition between these genomic elements. The calculation of the mitotic instability index implied that B chromosomes in this species might be in a final stage of instability.

引用

页码：141 / 151

页数：11

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