Measurement of ciliary beating and fluid flow in the zebrafish adult telencephalon

被引：4

作者：

Jeong, Inyoung ^{[1
]}

Hansen, Jan Niklas ^{[2
,3
]}

Wachten, Dagmar ^{[2
]}

Jurisch-Yaksi, Nathalie ^{[1
]}

机构：

[1] Norwegian Univ Sci & Technol, Dept Clin & Mol Med, Erling Skjalgsons Gate 1, N-7491 Trondheim, Norway

[2] Univ Bonn, Inst Innate Immun, Med Fac, Biophys Imaging, D-53127 Bonn, Germany

[3] KTH Royal Inst Technol, Sch Engn Sci Chem Biotechnol & Hlth, Sci Life Lab, S-17121 Stockholm, Sweden

来源：

STAR PROTOCOLS | 2022年 / 3卷 / 03期

基金：

芬兰科学院;

关键词：

TEMPERATURE; PLATFORM; NASAL;

D O I：

10.1016/j.xpro.2022.101542

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Motile cilia are hair-like structures that move and propel fluid, playing important roles in the physiology of organs. Here, we present a protocol to visualize and measure ciliary beating and cerebrospinal fluid (CSF) flow in the telencephalon of an adult zebrafish brain explant. We describe the preparation of brain ex plants, the recording of ciliary beating and CSF flow, and data analysis using ImageJ and MATLAB. These imaging and analysis techniques can be directly translated to other ciliated systems. For complete details on the use and execution of this protocol, please refer to D'Gama et al. (2021).

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页数：27

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