Population balance modelling of stem cell culture in 3D suspension bioreactors

被引：13

作者：

Bartolini, Edoardo ^{[1
]}

Manoli, Harry ^{[1
]}

Costamagna, Eleonora ^{[1
]}

Jeyaseelan, Hari Athitha ^{[1
]}

Hamad, Mouna ^{[1
]}

Irhimeh, Mohammad R. ^{[2
,3
]}

Khademhosseini, Ali ^{[4
,5
,6
]}

Abbas, Ali ^{[1
]}

机构：

[1] Univ Sydney, Sch Chem & Biomol Engn, Sydney, NSW 2006, Australia

[2] Univ Western Australia, Fac Med Dent & Hlth Sci, Perth, WA 6009, Australia

[3] Royal Perth Hosp, Cell & Tissue Therapies WA, Perth, WA 6000, Australia

[4] Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA

[5] Harvard Univ, Sch Med, Biomat Innovat Res Ctr, Biomed Engn Div,Dept Med,Brigham & Womens Hosp, Boston, MA 02139 USA

[6] MIT, Harvard MIT Div Hlth Sci & Technol, Cambridge, MA 02139 USA

来源：

CHEMICAL ENGINEERING RESEARCH & DESIGN | 2015年 / 101卷

关键词：

Stem cells; Bioreactor; Population balance; Mathematical modelling; GROWTH; DIFFERENTIATION; EXPANSION;

D O I：

10.1016/j.cherd.2015.07.014

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Growing and culturing stem cells in scalable quantities is of significant interest for both research and therapy. In this paper, a computational population balance model is developed and validated for the description of specified inter/intra-cellular related stem cell properties of interest (e.g. cell number, mass, size, age). The model describes the effects of extra-cellular variables; (A) bioreactor batch mode initial concentrations (substrate (1.5-5 g/l), dissolved oxygen (hypoxic (5%), normoxic (21%), and hyperoxic (35%)), (B) inoculum (3.75 x 10(4)-5.0 x 10(5) cells/ml), and (C) fed-batch mode conditions (media feed rate (10-100 ml/h) and air inlet flow rate 0.5-5l/min). The highest cell concentration achieved within the analysed simulation variable ranges is found to be approximately 1.9 x 10(6) cells/ml. Importantly, the results imply the existence of an optimum (maximum) cell concentration within the extra-cellular variable space, and identifying such optimality is non-trivial and would require a computational optimisation approach for this stem cell bioreactor process. (C) 2015 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.

引用

页码：125 / 134

页数：10

共 40 条

[1]

[Anonymous], 1932, Theoretische Biologie

[2] Development of a perfusion fed bioreactor for embryonic stem cell-derived cardiomyocyte generation: Oxygen-mediated enhancement of cardiomyocyte output [J].

Bauwens, C ;

Yin, T ;

Dang, S ;

Peerani, R ;

Zandstra, PW .

BIOTECHNOLOGY AND BIOENGINEERING, 2005, 90 (04) :452-461

[3]

Becker S., 2012, CELLS, V1

[4] A modular simulation package for fed-batch fermentation:: penicillin production [J].

Birol, G ;

Ündey, C ;

Çinar, A .

COMPUTERS & CHEMICAL ENGINEERING, 2002, 26 (11) :1553-1565

[5] Differentiation of pluripotent embryonic stem cells into cardiomyocytes [J].

Boheler, KR ;

Czyz, J ;

Tweedie, D ;

Yang, HT ;

Anisimov, SV ;

Wobus, AM .

CIRCULATION RESEARCH, 2002, 91 (03) :189-201

[6]

Carcano S., 2010, THESIS POLITECNICO M

[7]

Chaudhuri J., 2005, BIOREACTORS TISSUE E

[8]

Costamagna E., 2013, MODELLING HEMATOPOIE

[9] The Dual Arrhenius and Michaelis-Menten kinetics model for decomposition of soil organic matter at hourly to seasonal time scales [J].

Davidson, Eric A. ;

Samanta, Sudeep ;

Caramori, Samantha S. ;

Savage, Kathleen .

GLOBAL CHANGE BIOLOGY, 2012, 18 (01) :371-384

[10] Reconstruction of the oxygen uptake and carbon dioxide evolution rates of microbial cultures at near-neutral pH during highly dynamic conditions [J].

de Jonge, L. P. ;

Heijnen, J. J. ;

van Gulik, W. M. .

BIOCHEMICAL ENGINEERING JOURNAL, 2014, 83 :42-54

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