Fingerprint Analysis and Simultaneous Determination of Phenolic Compounds in Extracts of Curculiginis Rhizoma by HPLC-Diode Array Detector

被引：16

作者：

Bian, Qingya ^{[1
]}

Yang, Hui ^{[2
]}

Chan, Chi-on ^{[2
]}

Jin, Dengping ^{[2
]}

Mok, Daniel Kam-Wah ^{[2
]}

Chen, Sibao ^{[2
,3
,4
]}

机构：

[1] Yancheng Inst Hlth Sci, Yancheng 224005, Peoples R China

[2] Hong Kong Polytech Univ, State Key Lab Incubat Base Chinese Med & Mol Phar, Dept Appl Biol & Chem Technol, Shenzhen 518057, Peoples R China

[3] Chinese Acad Med Sci, Inst Med Plant Dev, Beijing 100193, Peoples R China

[4] Peking Union Med Coll, Beijing 100193, Peoples R China

来源：

CHEMICAL & PHARMACEUTICAL BULLETIN | 2013年 / 61卷 / 08期

基金：

中国国家自然科学基金;

关键词：

Curculiginis Rhizoma; phenolic compound; HPLC fingerprint; Curculigo orchioides; curculigoside; CHROMATOGRAPHIC FINGERPRINT; ORCHIOIDES; GLYCOSIDES; CURCULIGOSIDE;

D O I：

10.1248/cpb.c12-01058

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Curculiginis Rhizoma (Curculigo orchioides GAERTN.) is a well-known Chinese herbal medicine, as well as an important Rasayana drug in India. Current criteria of quality control on this herb are to quantitatively analyze single compound curculigoside, which fail to comprehensively evaluate quality of this herb. In this paper, a simple and reliable HPLC coupled with diode array detector (DAD) method was developed to evaluate the quality of Curculiginis Rhizoma through establishing chromatographic fingerprint and simultaneously quantitating four phenolic compounds, orcinol glucoside, orcinol, 2,6-dimethoxybenzoic acid and curculigoside. The fingerprint displayed eleven common peaks, and the similarity index of different samples was in a range of 0.890-0.977. Validation of the method was acceptable, with 96.03-102.82% accuracy in recovery test and inter and intra-day precisions were less than 2%. This developed method by having a combination of chromatographic fingerprint and quantitation analysis could be applied to the quality control of Curculiginis Rhizoma.

引用

页码：802 / 808

页数：7

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