The non-functional ACE2 isoform, but not the SARS-CoV-2 receptor, is induced as an interferon-stimulated gene, in SARS-CoV-2 infected adults

被引：1

作者：

Oliveto, Giuseppe ^{[1
]}

Scagnolari, Carolina ^{[1
]}

Frasca, Federica ^{[1
]}

Sorrentino, Leonardo ^{[1
]}

Matera, Luigi ^{[2
]}

Nenna, Raffaella ^{[2
]}

Viscido, Agnese ^{[1
]}

Scordio, Mirko ^{[1
]}

Petrarca, Laura ^{[2
]}

Zicari, Anna Maria ^{[2
]}

Gentilini, Elio ^{[3
]}

'Ettorre, Gabriella ^{[3
]}

Ceccarelli, Giancarlo ^{[3
]}

Midulla, Fabio ^{[2
]}

Antonelli, Guido ^{[1
]}

Pierangeli, Alessandra ^{[1
]}

机构：

[1] Sapienza Univ, Dept Mol Med, Virol Lab, Rome, Italy

[2] Sapienza Univ, Dept Maternal Infantile & Urol Sci, Rome, Italy

[3] Sapienza Univ, Dept Publ Hlth & Infect Dis, Rome, Italy

来源：

CYTOKINE | 2022年 / 158卷

基金：

欧盟地平线“2020”;

关键词：

ACE2; deleted ACE2; SARS-CoV-2; COVID-19; Interferon; ISG15;

D O I：

10.1016/j.cyto.2022.155997

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The recently discovered truncated, non-functional, ACE2 transcript (dACE2), but not the full-length ACE2 (f-lACE2), is induced by IFNs in differentiated airway cells. We measured expression of both ACE2 isoforms in SARS-CoV-2 positive and negative subjects, in relation to Interferon-stimulated genes. A significant activation of dACE2 transcript was found, in SARS-CoV-2 positive adults either hospitalized or not, showing a positive cor-relation with ISG15; f-lACE2 expression was weakly activated and not ISG-related. We confirmed a specific activation of dACE2 transcript in nasopharyngeal cells, related to the mucosal IFN response.

引用

页数：4

共 15 条

[1] A novel ACE2 isoform is expressed in human respiratory epithelia and is upregulated in response to interferons and RNA respiratory virus infection [J].

Blume, Cornelia ;

Jackson, Claire L. ;

Spalluto, Cosma Mirella ;

Legebeke, Jelmer ;

Nazlamova, Liliya ;

Conforti, Franco ;

Perotin, Jeanne-Marie ;

Frank, Martin ;

Butler, John ;

Crispin, Max ;

Coles, Janice ;

Thompson, James ;

Ridley, Robert A. ;

Dean, Lareb S. N. ;

Loxham, Matthew ;

Reikine, Stephanie ;

Azim, Adnan ;

Tariq, Kamran ;

Johnston, David A. ;

Skipp, Paul J. ;

Djukanovic, Ratko ;

Baralle, Diana ;

McCormick, Christopher J. ;

Davies, Donna E. ;

Lucas, Jane S. ;

Wheway, Gabrielle ;

Mennella, Vito .

NATURE GENETICS, 2021, 53 (02) :205-+

[2] Immunology of SARS-CoV-2 infection in children [J].

Chou, Janet ;

Thomas, Paul G. ;

Randolph, Adrienne G. .

NATURE IMMUNOLOGY, 2022, 23 (02) :177-185

[3] ACE2: Evidence of role as entry receptor for SARS-CoV-2 and implications in comorbidities [J].

Cuervo, Natalia Zamorano ;

Grandvaux, Nathalie .

ELIFE, 2020, 9

[4] Differential Downregulation of ACE2 by the Spike Proteins of Severe Acute Respiratory Syndrome Coronavirus and Human Coronavirus NL63 [J].

Glowacka, Ilona ;

Bertram, Stephanie ;

Herzog, Petra ;

Pfefferle, Susanne ;

Steffen, Imke ;

Muench, Marcus O. ;

Simmons, Graham ;

Hofmann, Heike ;

Kuri, Thomas ;

Weber, Friedemann ;

Eichler, Jutta ;

Drosten, Christian ;

Poehlmann, Stefan .

JOURNAL OF VIROLOGY, 2010, 84 (02) :1198-1205

[5] SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor [J].

Hoffmann, Markus ;

Kleine-Weber, Hannah ;

Schroeder, Simon ;

Krueger, Nadine ;

Herrler, Tanja ;

Erichsen, Sandra ;

Schiergens, Tobias S. ;

Herrler, Georg ;

Wu, Nai-Huei ;

Nitsche, Andreas ;

Mueller, Marcel A. ;

Drosten, Christian ;

Poehlmann, Stefan .

CELL, 2020, 181 (02) :271-+

[6] JAK inhibitors dampen activation of interferon-stimulated transcription of ACE2 isoforms in human airway epithelial cells [J].

Lee, Hye Kyung ;

Jung, Olive ;

Hennighausen, Lothar .

COMMUNICATIONS BIOLOGY, 2021, 4 (01)

[7] Alteration of L-Dopa decarboxylase expression in SARS-CoV-2 infection and its association with the interferon-inducible ACE2 isoform [J].

Mpekoulis, George ;

Frakolaki, Efseveia ;

Taka, Styliani ;

Ioannidis, Anastasios ;

Vassiliou, Alice G. ;

Kalliampakou, Katerina, I ;

Patas, Kostas ;

Karakasiliotis, Ioannis ;

Aidinis, Vassilis ;

Chatzipanagiotou, Stylianos ;

Angelakis, Emmanouil ;

Vassilacopoulou, Dido ;

Vassilaki, Niki .

PLOS ONE, 2021, 16 (06)

[8] Tissue-specific and interferon-inducible expression of nonfunctional ACE2 through endogenous retroelement co-option [J].

Ng, Kevin W. ;

Attig, Jan ;

Bolland, William ;

Young, George R. ;

Major, Jack ;

Wrobel, Antoni G. ;

Gamblin, Steve ;

Wack, Andreas ;

Kassiotis, George .

NATURE GENETICS, 2020, 52 (12) :1294-1302

[9] Interferons and viruses induce a novel truncated ACE2 isoform and not the full-length SARS-CoV-2 receptor [J].

Onabajo, Olusegun O. ;

Banday, A. Rouf ;

Stanifer, Megan L. ;

Yan, Wusheng ;

Obajemu, Adeola ;

Santer, Deanna M. ;

Florez-Vargas, Oscar ;

Piontkivska, Helen ;

Vargas, Joselin M. ;

Ring, Timothy J. ;

Kee, Carmon ;

Doldan, Patricio ;

Tyrrell, D. Lorne ;

Mendoza, Juan L. ;

Boulant, Steeve ;

Prokunina-Olsson, Ludmila .

NATURE GENETICS, 2020, 52 (12) :1283-1293

[10] ACE2 Expression Is Increased in the Lungs of Patients With Comorbidities Associated With Severe COVID-19 [J].

Pinto, Bruna G. G. ;

Oliveira, Antonio E. R. ;

Singh, Youvika ;

Jimenez, Leandro ;

Goncalves, Andre N. A. ;

Ogava, Rodrigo L. T. ;

Creighton, Rachel ;

Schatzmann Peron, Jean Pierre ;

Nakaya, Helder, I .

JOURNAL OF INFECTIOUS DISEASES, 2020, 222 (04) :556-563

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