Arabinose substitution degree in xylan positively affects lignocellulose enzymatic digestibility after various NaOH/H2SO4 pretreatments in Miscanthus

被引：111
作者：
Li, Fengcheng [1 ,2 ,3 ,4 ]
Ren, Shuangfeng [1 ,2 ,3 ,4 ]
Zhang, Wei [1 ,2 ,3 ,5 ]
Xu, Zhengdan [1 ,2 ,3 ,4 ]
Xie, Guosheng [1 ,2 ,3 ,4 ]
Chen, Yan [1 ,2 ,3 ,4 ]
Tu, Yuanyuan [1 ,2 ,3 ,4 ]
Li, Qing [3 ,6 ,12 ]
Zhou, Shiguang [3 ,12 ]
Li, Yu [1 ,2 ,3 ,4 ]
Tu, Fen [1 ,2 ,3 ,5 ]
Liu, Lin [1 ,2 ,3 ,4 ]
Wang, Yanting [1 ,2 ,3 ,4 ]
Jiang, Jianxiong [7 ]
Qin, Jingping [7 ]
Li, Shizhong [8 ]
Li, Qiwei [9 ]
Jing, Hai-Chun [10 ]
Zhou, Fasong [11 ]
Gutterson, Neal [11 ]
Peng, Liangcai [1 ,2 ,3 ,4 ,5 ]
机构：
[1] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China
[2] Huazhong Agr Univ, Natl Ctr Plant Gene Res Wuhan, Wuhan 430070, Peoples R China
[3] Huazhong Agr Univ, Biomass & Bioenergy Res Ctr, Wuhan 430070, Peoples R China
[4] Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China
[5] Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Peoples R China
[6] Huazhong Agr Univ, Coll Sci, Wuhan 430070, Peoples R China
[7] Hunan Agr Univ, Dept Biotechnol, Changsha 410128, Hunan, Peoples R China
[8] Tsinghua Univ, Inst New Energy Technol, Beijing 100084, Peoples R China
[9] Guangzhou Sugarcane Ind Res Inst, Guangdong Key Lab Sugarcane Improvement & Biorefi, Guangzhou 510316, Guangdong, Peoples R China
[10] Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
[11] Mendel Biotechnol Inc, Hayward, CA 94545 USA
[12] Hubei Aohua Bioenergy Ind Corp Ltd, Hanchuan 431602, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 2013年 / 130卷
关键词：
Miscanthus; Xylan; Biomass digestibility; Cellulases; Chemical pretreatment; DILUTE-ACID PRETREATMENT; BIOFUEL PRODUCTION; WHEAT-STRAW; CORN STOVER; CELL-WALLS; BIOMASS; HYDROLYSIS; CELLULOSE; LIGNIN; HEMICELLULOSES;
D O I：
10.1016/j.biortech.2012.12.107
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
Xylans are the major hemicelluloses in grasses, but their effects on biomass saccharification remain unclear. In this study, we examined the 79 representative Miscanthus accessions that displayed a diverse cell wall composition and varied biomass digestibility. Correlation analysis showed that hemicelluloses level has a strong positive effect on lignocellulose enzymatic digestion after NaOH or H2SO4 pretreatment. Characterization of the monosaccharide compositions in the KOH-extractable and non-KOH-extractable hemicelluloses indicated that arabinose substitution degree of xylan is the key factor that positively affects biomass saccharification. The xylose/arabinose ratio after individual enzyme digestion revealed that the arabinose in xylan is partially associated with cellulose in the amorphous regions, which negatively affects cellulose crystallinity for high biomass digestibility. The results provide insights into the mechanism of lignocellulose enzymatic digestion upon pretreatment, and also suggest a goal for the genetic modification of hemicelluloses towards the bioenergy crop breeding of Miscanthus and grasses. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：629 / 637
页数：9
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