A new method to quantify phagocytosis and intracellular degradation using green fluorescent protein-labeled Escherichia coli:: Comparison of cord blood macrophages and peripheral blood macrophages of healthy adults

被引：43

作者：

Gille, C ^{[1
]}

Spring, B ^{[1
]}

Tewes, L ^{[1
]}

Poets, CF ^{[1
]}

Orlikowsky, T ^{[1
]}

机构：

[1] Univ Childrens Hosp, Dept Neonatol, D-72070 Tubingen, Germany

来源：

CYTOMETRY PART A | 2006年 / 69A卷 / 03期

关键词：

macrophages-1; phagocytosis; subpopulations; cord blood; GFP; FACS;

D O I：

10.1002/cyto.a.20222

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Interactions between innate and adaptive immune functions in neonatal macrophages (M Phi) are still unclear. We therefore established a method to quantify bacterial phagocytosis and intracellular degradation, using green fluorescent protein (GFP)-labeled Escherichia coli in combination with phenotypic analysis. The kinetics of the proportion of phagocyting M Phi, phagocytosed bacteria per M Phi, and bacterial degradation were comparable for cord blood M Phi of term neonates and M Phi of healthy adults. Phenotyping revealed CD14 and CD16 to be down-modulated within minutes. GFP-labeted E. coli may be useful tools to further study M Phi subpopulations and determinants of phagocytosis in cord blood M Phi. (c) 2006 International Society for Analytical Cytology.

引用

页码：152 / 154

页数：3

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